高效液相色谱法测定番茄及其制品中番茄甙的含量  

Determination of tomatine in tomato and its processed products by high performance liquid chromatography

作　　者：张玉[1] 吴慧明[2] 王伟[1] 徐丽红[1] 王建清[1] 

机构地区：[1]浙江省农业科学院农产品质量标准研究所,浙江杭州310021 [2]浙江大学农药与环境毒理研究所,浙江杭州310029

出　　处：《食品工业科技》2012年第15期322-324,共3页Science and Technology of Food Industry

基　　金：浙江省科技厅重大科技专项(2009C12052)

摘　　要：建立了固相萃取-高效液相色谱(HPLC)测定番茄及番茄制品中番茄甙的方法。样品中的番茄甙经过甲醇提取,PCX固相萃取法净化,乙酰化后,采用高效液相色谱法进行测定。以乙腈和水(90:10)为流动相,经过C18色谱柱分离,紫外检测器检测。结果表明:番茄甙的检出限为0.5mg/kg,方法回收率为90.5%～93.5%,相对标准差为1.7%～3.2%。该方法准确、灵敏、适用于番茄及番茄制品中番茄甙的测定。A method was developed for determination of tomatine in tomato and its processed products by high performance liquid chromatography（HPLC）.The tomatine in sample was extracted by methanol,cleaned up with PCX solid-phase extraction cartridge,then acetylated by acetic anhydride and determined by HPLC.The mobile phase consisted of 90% acetonitrile and 10% water.The tomatine was separated on C18 column and determined with UV detector at 205nm.The results showed：the detection limit of tomatine was 0.5mg/kg,the average recoveries were from 90.5% to 93.5%,and the relative standard deviations（RSDs）was from 1.7% to 3.2%.This method was accurate,sensitive and effective for determination of tomatine in tomato and its processed products.

关 键 词：番茄甙 测定 HPLC 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]