螺旋藻富铬及其含铬蛋白组份对α-葡萄糖苷酶抑制的研究  

Effects of chromium(Ⅲ)-riched Spirulina platensis proteins on the activity inhibition of α-glucosidase

作　　者：何颖敏[1] 孙宜君[2] 张娇[2] 李博生[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]北京林业大学螺旋藻研究所,北京100083

出　　处：《安徽农业大学学报》2012年第4期571-575,共5页Journal of Anhui Agricultural University

基　　金：教育部重点研究项目(102023);国家林业公益性行业科研专项(200704025)共同资助

摘　　要：在富铬Z氏培养液的条件下,研究了钝顶螺旋藻对Cr(Ⅲ)的吸收及其有机化程度,采用不同饱和度硫酸铵沉淀的方法分离提取不同蛋白组份,对其含Cr(Ⅲ)量进行了分析,并探讨了蛋白组份对α-葡萄糖苷酶活性的抑制作用。结果表明,螺旋藻培养液中CrCl3.6H2O添加浓度为300 mg·L-1,螺旋藻总铬富集量可达到3.30 mg·g-1,有机化程度为70.2%;不同饱和度(25%、50%和75%)硫酸铵沉淀出的蛋白组份中铬占总铬的情况分别为2.1%、16.8%和7.0%,不同蛋白质结合铬的能力各异;3种蛋白组份对α-葡萄糖苷酶均有抑制作用,其中50%饱和度硫酸铵沉淀的蛋白组份抑制活性最高,IC50为69.87 mg·mL-1。In this paper, we studied the absorption degree of Cr（Ⅲ） in Spirulina platensis which cultivated in chromium （Ⅲ）-riched Zarrouk medium, by extracting proteins with ammonium sulfate precipitation, analYzing the content of Cr（Ⅲ） in protein components. And then we investigated the inhibition effect of chromium （Ⅲ）-riched Spirulina platensis proteins on alpha-glucosidase. The results showed that Spirulina platensis growed in,culture medium added with 300 mg·L^1CrC13·6H2O, the total enriched Cr（Ⅲ） and it,; organic degree of Cr（Ⅲ） could reach to 3,30 mg·g^-1 and 70.2%, correspondingly. When different saturated solution （25%, 50% and 75%） of ammonium sulfate were used to precipitate proteins, the proportion of Cr（Ⅲ） in protein components to total Cr（Ⅲ） were 2.1%, 16.8% and 7.0%, respectively. The abilities of different proteins combined with Cr （Ⅲ） were different. The three protein components had different inhibition activities on alpha-glucosidase. Among them, protein com- ponents precipitated by 50% saturation solution of ammonium sulfate revealed the best inhibition effect on al- pha-glucosidase, with IC50 of 69.87 mg·mL^-1.

关 键 词：三价铬 螺旋藻蛋白 Α-葡萄糖苷酶 

分 类 号：Q949.22[生物学—植物学]