法医DNA标准物质STR位点等位基因分型定值研究  

Certified value study for alleles on STR loci of forensic DNA reference materials

作　　者：赵兴春[1] 孙敬[1] 印佳[2] 王燕[2] 姜伯玮 高运华[4] 叶健[1] 

机构地区：[1]公安部物证鉴定中心,北京100038 [2]中国人民公安大学,北京100038 [3]中科院应用物理研究所,上海201800 [4]中国计量科学研究院,北京100013

出　　处：《中国测试》2012年第4期55-57,共3页China Measurement & Test

基　　金：国家"十一五"科技支撑计划项目(2008BAK45B04)

摘　　要：为探讨法医DNA标准物质STR位点等位基因分型的定值和溯源,利用有机法提取法医DNA标准物质备选所用细胞基因组DNA,以其为模板进行STR复合扩增,制备获得等位基因分型片段并测序分析,成功获得法医DNA标准物质备选所用细胞STR位点等位基因分型的定值。对STR位点等位基因分型进行定值研究可以作为法医DNA标准物质溯源研究的有效途径之一。In order to develop the certified value and traceability for the alleles on STR loci of forensic DNA reference materials, the genomic DNA of the cells was extracted and amplified for preparing the forensic DNA reference materials. Then the allelic DNA was prepared, sequenced and valuated. Finally the value for the alleles on STR loci of the cells was certified. The certified value for the alleles on STR loci can work as an effective approach for the traceability of forensic DNA reference materials.

关 键 词：法医DNA 标准物质 STR位点 等位基因 定值 

分 类 号：Q783.1[生物学—分子生物学] Q755