快速成功建立兔腹主动脉狭窄的模型  

Successfully and quikly establishing atherosclerosis models of stenosis in the obdominal aorta in rabbits

作　　者：于洁[1] 高传玉[2] 许文克[2] 张连仲[3] 

机构地区：[1]郑州大学第一附属医院老年综合一科,450052 [2]郑州大学人民医院心血管科 [3]郑州大学人民医院超声科

出　　处：《中国实用医药》2012年第19期1-3,共3页China Practical Medicine

摘　　要：目的复制动脉粥样硬化狭窄的模型,血管造影测量动脉狭窄程度以证实造模成功。方法实验于2003年2～5月在郑州大学人民医院心血管内科实验室完成。选择雄性4月龄新西兰大白兔20只,随机分为单纯饲喂高脂饮食组、高脂饮食同时动脉内膜剥脱组,每组10只。分笼饲养,饲喂正常的全价颗粒饲料,自由饮水,观察1周后进入实验。1周后所有的兔子均开始高脂饮食(含1.5%胆固醇)喂养,高脂饮食同时动脉内膜剥脱组高脂喂养3d后用股动脉穿刺法行腹主动脉内皮剥脱术。术后继续喂养同样的高脂饲料。于术后第9周行腹主动脉血管造影检查,记录腹主动脉狭窄程度。结果术后第9周进行血清总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白胆固醇测定,两组兔血清总胆固醇、甘油三酯、高、低密度脂蛋白胆固醇均升高,高脂饮食与动脉内膜剥脱组升高更明显(P<0.05)。血管造影显示所有兔的动脉粥样硬化狭窄均形成,目测狭窄程度在50%～70%[(57.000±7.888)%],狭窄长度8～23mm。结论动脉内膜剥脱术同时饲喂高胆固醇饮食可以在短时间内复制出动脉粥样硬化狭窄模型,血管造影判定血管狭窄程度。Objective To establish models of atherosclerosis and stenosis of rabbits, and evaluate the degree of stenosis by angiography.Methods The experiment was carried out in the laboratory of Department of Cardiovascular Medicine, People’s Hospital of Zhengzhou University from February to May 2003.Twenty male New Zealand rabbits aged 4 months were fed with a standard diet in separate cages for 1 week without inhibiting drinking. Twenty New Zealand rabbits were randomly divided into high-fat diet and high-fat diet combined with arterial injury groups with 10 animals in each group.One week later, all the rabbits were fed with cholesterol-supplemented diet （1.5% cholesterol） and rabbits of high-fat diet combined with arterial injury groups were subject to balloon catheter injury of the abdominal aorta by puncturing into femoral artery after 3 days of cholesterol-fed. Rabbits were continuing fed with cholesterol-rich diet after the operation. Angiography were used to record the degree of stenosis of the abdominal aorta at the 9th week after operation.Results All the 20 rabbits entered the result analysis. ①After 9 weeks,the levels of TC,TG,HDL,LDL were increased,which was more obviously in the high-fat diet combined with arterial injury groups. ② Stenosis of abdominal aorta was observed at the 9th week by angiography and the rate of stenosis was 50%~70% [（57.000±7.888）%] and it was 8~23 mm in length. Conclusion The animal model of stenosis can be successfully established in a short time by balloon endothelial denudation of abdominal aorta combined with cholesterol-fed. Angiography can accurately measure the degree of stenosis of the abdominal aorta.

关 键 词：膳食 动脉粥样硬化 动物模型 血管造影术 

分 类 号：R-332[医药卫生]