两种粤产粉孢牛肝菌抗氧化活性  

Antioxidant Activity of Aqueous and Aqueous-Ethanolic Extracts of Two Tylopilus Species Endemic in Guangdong Province,China

作　　者：张明[1,2] 闫文娟[2] 李泰辉[2] 宋斌[2] 图力古尔[1] 

机构地区：[1]吉林农业大学,吉林长春130118 [2]广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东省华南应用微生物重点实验室-省部共建国家重点实验室培育基地,广东省微生物应用新技术公共实验室,广东广州510070

出　　处：《食用菌学报》2012年第2期91-94,共4页Acta Edulis Fungi

基　　金：国家基金项目(编号:30970023);广东省科学院人才基金[编号:粤科优(2008)01];广东省科学院野外台站项目(编号:sytz201008)的部分研究内容

摘　　要：研究两种产自广东的粉孢牛肝菌(Tylopilus)——类铅紫粉孢牛肝菌(T.plumbeoviolaceoides)和花盖粉孢牛肝菌(GDGM27210)的子实体水提液和75%乙醇提取液的体外抗氧化活性。结果表明,在实验浓度范围内,两种牛肝菌的子实体水提液和75%乙醇提液有不同程度的抑制亚油酸自氧化和清除自由基DPPH.、羟自由基.OH的作用。其中水提液对抑制亚油酸自氧化作用效果较好,最大抑制率分别可达95.74%和92.79%;而75%乙醇提取液对清除DPPH.效果较好,最大清除率分别可达86.68%和94.03%。Aqueous and 75% ethanolic extracts of fruit bodies of two Tylopilus species endemic in Guangdong Province were evaluated for in vitro antioxidant activity using free-radical scavenging-and lipid peroxidation-based assays.Extracts of Tylopilus plumbeoviolaceoides and Tylopilus sp.GDGM27210 inhibited autoxidation of linoleic acid and exhibited DPPH·and ·OH scavenging activity.Aqueous extracts of T.plumbeoviolaceoides and Tylopilus sp.GDGM27210 inhibited linoleic acid oxidation by approximately 45%-95% and 26%-93%,respectively over the concentration range 0.1-1.0 mg/mL,while the corresponding values for 75% ethanolic extracts were 80%-98% and 41%-88%.Over the same extract concentration range,·OH radical scavenging activity of aqueous extracts of T.plumbeoviolaceoides and Tylopilus sp.GDGM27210 ranged between 12.5%-85% and 8.0%-54% respectively,whereas the corresponding values for 75% ethanolic extracts were 17%-72% and 10%-41%.DPPH· radical scavenging activity of aqueous extracts（0.5-10.0 mg/mL） of T.plumbeoviolaceoides and Tylopilus sp.GDGM27210 ranged between 38%-90% and 55%-97%,respectively.DPPH· radical scavenging activity of 75% aqueous ethanolic extracts（0.1-2.0 mg/mL） of T.plumbeoviolaceoides and Tylopilus sp.GDGM27210 ranged between 21%-87% and 48%-94%,respectively.

关 键 词：牛肝菌 抗氧化活性 自由基 

分 类 号：R371[医药卫生—病原生物学]