Vitamin D deficiency: Correlation to interleukin-17, interleukin-23 and PⅢNP in hepatitis C virus genotype 4  

作　　者：Mona F Schaalan Waleed A Mohamed Hesham H Amin 

机构地区：[1]Department of Biochemistry, Faculty of Pharmacy, Misr International University, Cairo 1, Egypt [2]Department of Chemistry, Cairo University, Cairo 11562, Egypt [3]Department of Clinical Pathology, Faculty of Medicine, AL Azhar University, Cairo 147, Egypt

出　　处：《World Journal of Gastroenterology》2012年第28期3738-3744,共7页世界胃肠病学杂志（英文版）

摘　　要：AIM: To assess vitamin D (Vit D) abnormalities in hepatitis C infected patients and their relationship with interleukin (IL)-17, IL-23 and N-terminal propeptide of type Ⅲ pro-collagen (PⅢNP) as immune response mediators. METHODS: The study was conducted on 50 Egyptian patients (36 male, 14 female) with hepatitis C virus (HCV) infection, who visited the Hepatology Outpatient Clinic in the Endemic Disease Hospital at Cairo University. Patients were compared with 25 ageand sexmatched healthy individuals. Inclusion criteria were based on a history of liver disease with HCV genotype 4 (HCV-4) infection (as new patients or under followup). Based on ultrasonography, patients were classified into four subgroups; 14 with bright hepatomegaly; 11 with perihepatic fibrosis; 11 with hepatic cirrhosis; and 14 with cirrhosis and hepatocellular carcinoma (HCC).Total Vit D (i.e., 25-OH-Vit D) and active Vit D [i.e., 1,25-(OH) 2 -Vit D] assays were carried out using commercial kits. IL-17, IL-23 and PⅢNP levels were assayed using enzyme linked immunosorbent assay kits, while HCV virus was measured by quantitative and qualitative polymerase chain reaction. RESULTS: Levels of Vit D and its active form were significantly lower in advanced liver disease (hepatic cirrhosis and/or carcinoma) patients, compared to those with bright hepatomegaly and perihepatic fibrosis. IL-17, IL-23 and PⅢNP levels were markedly increased in HCV patients and correlated with the progression of hepatic damage. The decrease in Vit D and active Vit D was concomitant with an increase in viral load, as well as levels of IL-17, IL-23 and PⅢNP among all subgroups of HCV-infected patients, compared to normal healthy controls. A significant negative correlation was evident between active Vit D and each of IL-17, IL-23 and PⅢNP (r = -0.679, -0.801 and -0.920 at P < 0.001, respectively). HCV-infected men and women showed no differences with respect to Vit D levels. The viral load was negatively correlated with Vit D and active Vit D (r = -0.084 and -0.84AIM： To assess vitamin D （Vit D） abnormalities in hepatitis C infected patients and their relationship with interleukin （IL）-17, IL-23 and N-terminal propeptide of type Ⅲ pro-collagen （PⅢNP） as immune response mediators. METHODS： The study was conducted on 50 Egyptian patients （36 male, 14 female） with hepatitis C virus （HCV） infection, who visited the Hepatology Outpatient Clinic in the Endemic Disease Hospital at Cairo University. Patients were compared with 25 ageand sexmatched healthy individuals. Inclusion criteria were based on a history of liver disease with HCV genotype 4 （HCV-4） infection （as new patients or under followup）. Based on ultrasonography, patients were classified into four subgroups; 14 with bright hepatomegaly; 11 with perihepatic fibrosis; 11 with hepatic cirrhosis; and 14 with cirrhosis and hepatocellular carcinoma （HCC）.Total Vit D （i.e., 25-OH-Vit D） and active Vit D [i.e., 1,25-（OH） 2 -Vit D] assays were carried out using commercial kits. IL-17, IL-23 and PⅢNP levels were assayed using enzyme linked immunosorbent assay kits, while HCV virus was measured by quantitative and qualitative polymerase chain reaction. RESULTS： Levels of Vit D and its active form were significantly lower in advanced liver disease （hepatic cirrhosis and/or carcinoma） patients, compared to those with bright hepatomegaly and perihepatic fibrosis. IL-17, IL-23 and PⅢNP levels were markedly increased in HCV patients and correlated with the progression of hepatic damage. The decrease in Vit D and active Vit D was concomitant with an increase in viral load, as well as levels of IL-17, IL-23 and PⅢNP among all subgroups of HCV-infected patients, compared to normal healthy controls. A significant negative correlation was evident between active Vit D and each of IL-17, IL-23 and PⅢNP （r = -0.679, -0.801 and -0.920 at P 0.001, respectively）. HCV-infected men and women showed no differences with respect to Vit D levels. The viral load was negatively correlat

关 键 词：Vitamin D INTERLEUKIN-17 INTERLEUKIN-23 N-terminal propeptide of type Ⅲ pro-collagen Hepatitis genotype 4 

分 类 号：R512.63[医药卫生—内科学]