分批补料及缺氮培养对小球藻油脂产量的影响  

Effects of Fed-batch Cultivation and Nitrogen Deficiency on Lipid Yield of Chlorella vulgaris

作　　者：葛珍珍[1] 王杰[1] 余晓斌[1] 

机构地区：[1]江南大学生物工程学院,工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《安徽农业科学》2012年第24期11929-11931,11971,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为了实现小球藻的高密度及高产油培养。[方法]通过分析分批培养过程中藻细胞的生长曲线、葡萄糖消耗曲线、pH及溶氧变化曲线,对小球藻进行分批补料,待藻细胞达到一定的高密度后再进行缺氮培养以富集细胞内的油脂。[结果]经过4次分批补料,小球藻的生物量达到了65.25 g/L,然后进行缺氮培养12 h,小球藻的油脂含量由42.75%提高到了63.82%,油脂产量达43.37 g/L。[结论]合理的分批补料明显地提高了小球藻的生物量。缺氮培养进一步提高了小球藻的油脂含量。[ Objective ] To achieve high yield of biomass and lipid of Chlorella vulgaris. [ Method ] The growth curve of C. vulgaris, variation curves of residual glucose concentration, pH, dissolved oxygen of the medium during batch cultivation were analyzed to culture C. vulgaris accordingly by fed-batch until certain density, and then the C. vulgaris was cultured with deficient nitrogen to enrich the lipid. [ Result] After fed-batch cultivation for four times, the biomass yield of C. vulgaris reached 65.25 g/L, then cultured by nitrogen deficiency for another 12 hours, the lipid content was improved from 42.75% to 63.82% , and the lipid yield reached 43.37 g/L. [ Conclusion] Reasonable fed-batch culture greatly improves the biomass of C. vulgaris, while the cultivation by nitrogen deficiency further increases its lipid content.

关 键 词：小球藻 分批补料 缺氮培养 油脂含量 

分 类 号：S182[农业科学—农业基础科学]