海黍子石油醚相提取物的抑菌和免疫活性的研究  

Antibacterial and immune activity of petroleum ether extract phase from the Sargassum kjellmanianu

作　　者：李丽[1] 曲雁 曹务波 马伟伟[1] 周革非[1] 

机构地区：[1]烟台大学生命科学学院,烟台264005 [2]烟台市产品质量监督检验所,烟台264003 [3]莱阳翰霖生物技术有限公司,莱阳265200

出　　处：《食品科技》2012年第8期220-224,共5页Food Science and Technology

基　　金：山东省优秀中青年科学家科研奖励基金项目(2008BS06012);山东省自然科学基金项目(ZR2009BL030);烟台市科技发展计划项目(2008GGA0020080410084458)

摘　　要：为了研究海黍子不同提取物的抗菌和免疫活性,将海黍子乙醇提取物依次用石油醚、乙酸乙酯、正丁醇进行萃取。取石油醚相进行硅胶柱层析,分离得P1～P77种物质,并对其进行抑菌(金黄色葡萄球菌、枯草杆菌、大肠杆菌、面包酵母菌、假丝酵母菌)和免疫活性(小鼠脾淋巴细胞、腹腔巨噬细胞增殖实验和NO释放实验)研究。结果表明,这7种提取物对面包酵母菌和假丝酵母菌没有抑制作用,而对细菌(金黄色葡萄球菌,枯草杆菌和大肠杆菌)的抑制效果比较理想。免疫实验表明,石油醚相的7个样品都具有一定的免疫活性,不但可以促进小鼠巨噬细胞RAW264.7增殖和体外刺激淋巴细胞增殖,还可以促进小鼠腹腔巨噬细胞产生NO。因此海黍子具有一定的抑菌和免疫活性,值得深入研究。The ethanol extracts of the Sargassum kjellmanianum were extracted by the petroleum cetate and n-butanol successively. Then the antibacterial and immunity activity of different Sargassum kjellmanianum extracts were researched. Seven kinds of material （P1 to P7） were separated from the petroleum ether phase by the silica gel column chromatography. The research of their antibacterial and immunity activity were carried out. The fungus used in the antibacterial activity were the staphylococcus aurous, bacillus subtitles, escherichia coli, bread yeast and candida. Mice splenic lymphocytes value- added experiment and NO release experiment were carried out in the immunity activity research. The result shows that the seven extracts have no inhibition to the bread yeast and candida, and have good inhibition to the bacterium （staphylococcus aureus, bacillus subtitles and escherichia coli）. Results in immune experiments show that, each of the seven samples from the petroleum ether phase has certain of the immunity activity, which can not only promote the proliferation of mice RAW264.7 macrophage and the vitro stimulate lymphocyte, also can promote the mice abdominal macrophage to produce NO. As a result, the Sargassum kjeltmanianum has certain of the antibacterial and immunity activity, which is worth of researching.

关 键 词：海黍子 硅胶柱层析 抑菌活性 免疫活性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]