牛蒡根际可培养细菌多样性和镉耐性初步分析  

Priliminary Analysis of Cultivable Bacteria Diversity and Cd^(2+) Resistance in Burdock Rhizosphere

作　　者：侯进慧[1] 樊继强[1] 王富威[1] 蔡侃[1] 孔文刚[1] 

机构地区：[1]徐州工程学院食品(生物)工程学院,徐州221008

出　　处：《生物技术通报》2012年第8期158-162,共5页Biotechnology Bulletin

基　　金：徐州市科技计划项目(XZZD0924);徐州工程学院校级培育项目(XKY2011110);徐州工程学院2011年大学生创新创业基金项目

摘　　要：从牛蒡根际土壤中分离可培养细菌,进行多样性分析,并对镉耐受性菌株进行筛选及其抗性和种群多样性进行了分析。限制性内切酶多态性分析显示,分离的菌株可分为9个操作分类单元(OUT),分别属于变形菌门、厚壁菌门和放线菌门,分属于6个科,9个属,其中隶属于肠杆菌属、芽胞杆菌属和假单胞菌属的是优势物种。分离到的耐镉菌株分别属于Bacillus subtilis、Enterobacter aerogenes、Enterobacter ludwigi、Klebsiellasp.、Pectobacterium carotovorum、Pseudomonassp.,而Pectobacterium carotovorumNP22、Enterobacter ludwigii NP23、Pseudomonassp.NP39三菌株可在Cd2+浓度为400 mg/L固体培养基上生长。Cultivable bacterial strains were isolated from burdock rhizosphere, and genetic diversity and Cd2＋ resistance was analyzed with the isolated strains. Restriction enzyme morphology analysis demonstrates that the isolated 36 strains could be grouped into 9 different Operational Taxonomic Units （ OUTs ） . The isolated strains belong to 9 genera, 6 families of 3 major phylogenetic groups （ Proteobacteria, Firmicutes and Actinobacteria ） . Enterobacter, Bacillus and Pseudomonas are the dominant groups. The isolated Cd2＋ resistance strains were identified as Bacillus subtilis, Enterobacter aerogenes, Enterobacter ludwigi, Klebsiella sp., Pectobacterium carotovorum, Pseudomonas sp. and Pectobacte- rium carotovorum NP22, Enterobacter ludwigii NP23, Pseudomonas sp. NP39 have the highest MIC, which is 400 mg,/L Cd2＋on sold plate.

关 键 词：牛蒡 根际 镉 可培养细菌 多样性 

分 类 号：X173[环境科学与工程—环境科学]