分散液液微萃取-液相色谱法测定白葡萄酒中拟除虫菊酯类农药  

Determination of pyrethroid pesticide residues in white wine by disperse liquid-liquid microextraction coupled to high performance liquid chromatography

作　　者：周建科[1] 唐翠苓[1] 韩朝家[1] 李向梅[1] 

机构地区：[1]河北大学理化分析中心河北省分析科学与技术重点实验室,河北保定071002

出　　处：《中国酿造》2012年第7期164-166,共3页China Brewing

基　　金：国家计量基标准(化学部分)资源共享平台建设项目(2005DKAl0800)

摘　　要：分散液液微萃取法处理样品,结合高效液相色谱测定白葡萄酒中拟除虫菊酯类(氟氯氰菊酯、氯氰菊酯、氰戊菊酯、联苯菊酯)农药残留。二氯甲烷作萃取剂,乙醇作分散剂。色谱条件:反相C18色谱柱,甲醇∶乙腈∶水(10∶75∶15,体积比)为流动相,流速1.0mL/min,210nm紫外检测。在0.05μg/mL^50.00μg/mL范围内线性良好。相关系数均大于0.9993。平均回收率为83.20%~102.71%,相对标准偏差为1.47%~3.24%,检出限为1.00μg/L^2.00μg/L。A simple and efficient method using disperse liquid-liquid microextraction coupled to high performance liquid chromatography has been developed for the determination of pyrethroid pesticides（Cyfluthrin,Cypermethrin,Fenvalerateand and Bifenthrin） in White Wine.The wine sample which was contained in the tapered tube was extracted by dichloromethane,and the ethanol was dispersant.The four pesticides were separated by a Diamonsil C18column in the wavelength at 210nm.The mobile phase was methanol,acetonitrile,and water（10∶75∶15）,and flow rate was 1.0mL/min.A linear relationship was obtained for each analyte in the range of 0.05 and 50μg/mL with a correlation coefficiency（R）≥0.9993.The average recovery of the components ranged from 83.20% to 102.71%.The relative standard deviations（RSDs） were1.47%~3.24% and the determination limit was 1.00μg/L^2.00μg/L.

关 键 词：分散液液微萃取 高效液相色谱法 白葡萄酒 拟除虫菊酯 

分 类 号：O657.7[理学—分析化学]