正交实验法优化牛鼻软骨中硫酸软骨素的醇沉工艺  

Optimization of ethanol precipitation techniques for chondroitin sulfate from bovine nose cartilage with orthogonal test

作　　者：李利晓[1] 夏延斌[1,2] 

机构地区：[1]湖南农业大学食品科技学院,湖南长沙410128 [2]国家蔬菜加工技术研发分中心,湖南长沙410128

出　　处：《食品工业科技》2012年第16期261-264,共4页Science and Technology of Food Industry

基　　金：湖南省科技创新项目(60030183711)

摘　　要：研究牛鼻软骨碱提液中硫酸软骨素的醇沉工艺。通过单因素和正交实验对醇沉工艺进行优化,确定的最佳条件是:乙醇浓度65%,乙酸钠浓度4.5%,pH6.0。在该条件下,提取率为14.81%,葡萄糖醛酸含量33.36%,硫酸软骨素纯度达85.9%。紫外光谱分析:样品在196nm处有多糖的吸收峰,未见蛋白质(280nm)与核酸(260nm)的特征吸收峰。The ethanol deposition technology of chondroitin sulfate was studied in the paper,witch was extracted from bovine nose cartilage hydrolyzed by alkaline solution. The optimal ethanol deposition conditions of chondroitin sulfate were explored by single factor and orthogonal experiments to be ethanol solution of 65%, sodium acetate solution of 4.5% ,pH6.0. Under the optimal conditions,the yield was 14.81% ,the content of glucuronic acid was 33.36%,the purity quotient of chondroitin sulfate was 85.9%.UV analysis revealed that the absorption peak of chondroitin sulfate appears at 196nm,while characteristic absorption peaks of protein （280nm） and nucleic acid（260nm） were not found.

关 键 词：正交实验 硫酸软骨素 醇沉工艺 

分 类 号：TS201.1[轻工技术与工程—食品科学]