转野生荠菜凝集素基因棉花对赤子爱胜蚓的影响  

Effects of Wild-Shepherd's-Purse-Agglutinin(WSA) Transgenic Cotton on Eisenia foetida

作　　者：丁帅[1,2] 方志翔[1] 刘标[1] 沈文静[1] 孟军[1] 

机构地区：[1]环境保护部南京环境科学研究所国家环境保护生物安全重点实验室,江苏南京210042 [2]南京农业大学生命科学学院,江苏南京210095

出　　处：《生态与农村环境学报》2012年第4期389-393,共5页Journal of Ecology and Rural Environment

基　　金：转基因生物新品种培育科技重大专项(2011ZX08012-005;2011ZX08011-002)

摘　　要：在实验室条件下分别给赤子爱胜蚓(Eisenia foetida)饲喂在自然状况下可能接受的棉叶最高添加量和2倍最高添加量的转野生荠菜凝集素(WSA)基因棉花(Gossypium hirsutum)叶片,并以其亲本棉花叶片为对照,以牛粪为阴性对照,以农药为阳性对照,研究种植转WSA基因棉花对赤子爱胜蚓死亡率、生长及繁殖的影响。结果表明,在49 d的试验周期内,与取食亲本棉花叶片的赤子爱胜蚓相比,取食转WSA基因棉花叶片的赤子爱胜蚓的死亡率,体质量,GST、SOD、纤维素酶活性以及蚓茧数和小蚯蚓数均无显著差异,未发现转WSA基因棉花对赤子爱胜蚓有不利影响。Groups of earth worms（Eisenia foetida） were raised in laboratory.Group Ⅰ was fed with the highest naturally acceptable dose of wild shepherd’s purse agglutinin（WSA） transgenic cotton（Gossypium hirsutum） leaves,Group Ⅱ with twice as much,Group Ⅲ with non-transgenic parent cotton leaves as control,Group Ⅳ with cow dung as negative control and Group Ⅴ with pesticide as positive control.The objective of the study is to explore effects of planting WSA transgenic cotton on survival,growth,and reproduction of E.foetida.Results of the 49 d experiment show that there was no significant difference in death rate,weight,SOD activity,GST activity,cellulase activity,number of earthworm cocoons and number of baby earthworms between Group Ⅲ and Groups Ⅰ and Ⅱ,which suggests that WSA transgenic cotton does not have any significant adverse effects on E.foetida.

关 键 词：转野生荠菜凝集素基因棉花 赤子爱胜蚓 谷胱甘肽硫转移酶 超氧化物歧化酶 纤维素酶 繁殖 

分 类 号：X17[环境科学与工程—环境科学]