三氧化二镍诱导人肺成纤维细胞恶性转化的实验研究  

Study on the Malignant Transformation of Human Lung Fibroblasts Cell Induced by Nickel Sesquioxide

作　　者：刘艺敏[1] 张敬[1] 徐曼妮[1] 赵贤四[1] 

机构地区：[1]同济大学基础医学院预防医学教研室,上海200070

出　　处：《中国公共卫生》2000年第8期694-696,共3页Chinese Journal of Public Health

摘　　要：在体外用不同浓度的Ni2 O3 多次处理人肺成纤维细胞 (HLF) ,并进行转化细胞的恶性鉴定———刀豆凝集素A(ConA)试验和软琼脂细胞培养试验。发现Ni2 O3 可诱导HLF细胞恶性转化 ,转化细胞生长速度加快 ,排列紊乱 ,失去接触抑制 ,呈交叉重叠生长 ,在实验浓度范围内 ,转化率呈剂量—反应关系。转化细胞可被低浓度ConA凝集 ,并在软琼脂内生长。提示Ni2 O3 有较强的诱导人肺成纤维细胞恶性转化的能力 。In order to study effects of the malignant transformation of HLF cell induced by indissoluble Nickel sesquioxide.HLF cell was treated several time by Ni 2O 3 of different concentration in vitro.The identify of malignance on transformation HIF cell was carried out by the tests of ConA and semisolid agar culture.Ni 2O 3 can induce the malignant transfromation of HIF cell.The transformed cell proliferated rapidly.The transformed colonies,exhibited in extensive random orientation and the cells crossing-over.The frequency of transformation shows relation of dose-reaction in experimental concentration.The transformed cells could be agglutinated by lower concentration of ConA and could grow in semisold agear.There implied Ni 2O 3 can induce strongly malignant transformation of HLF cell.It may be carcinogenic to human.

关 键 词：二氧化二镍 细胞恶性转化 人肺成纤维细胞 

分 类 号：R73－34[医药卫生—肿瘤] R730.231.1[医药卫生—临床医学]