双岐杆菌对牙周病原菌的体外抗生作用  

The Antibiosis of Bifidobacterium on Periodontal Pathogens

作　　者：涂家珍[1] 张和光[1] 陈罕[1] 廖旭辉 杨国平[1] 

机构地区：[1]中山医科大学口腔医学院口腔内科,广东广州510055

出　　处：《中山医科大学学报》2000年第4期274-276,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：中山医科大学科研启动基金

摘　　要：【目的】探讨双岐杆菌DM 85 0 4对牙周病原菌的体外抗生作用。【方法】分别取每升 3× 10 8CFU (集落形成单位 ,colony formingunits,CFU)的牙龈卟啉单胞菌 (Pg) ,具核梭杆菌 (Fn) ,伴放线菌放线杆菌 (Aa) ,中间普氏菌 (Pi)菌液 2 0μL与每升 3× 10 10 CFU的DM 85 0 4菌液 2 0 μL混合培养 ,每株菌做 16支试管 ,以各菌的单独培养作对照 ,厌氧培养 36h ,平板菌落计数法定量测 DM85 0 4的抑菌作用 ,计算抑制率。【结果】 DM85 0 4对Pg 1312、Pg 332 77、Pi 2 5 2 6 1、Pi 2 5 6 11、Aa2 95 2 3、AaY4、Fn 10 95 3抑制率分别达到 5 5 42 %、5 7 2 1%、48 2 1%、46 6 9%、43 0 0 %、39 0 4%、49 36 %。双歧杆菌本身所受抑制作用则较弱 ,与Pg332 77混合培养的抑制率为 2 1 0 2 %。单因素方差分析表明抑制作用有非常显著的统计学意义 (P<0 0 1)。【结论】双岐杆菌DM 85 0 4对牙周致病菌Pg、Aa、Fn。Objective To investigate the antibiosis of Bifidobacterium DM 8504 on periodontal pathogens. Methods 20 μL of 3×10 8 CFU/L (colony forming units, CFU) Porphyromonas gingivalis (Pg), Fusobacterium nucleatun (Fn),Prevotella intermedius (Pi) and Actinobacillus actinomycetemcomitans  (Aa) were mixed with 20 μL of 3×10 10 CFU/L DM 8504 in 5 mL BHI broth test tubes respectively. Test tubes (n=16 for each bacterial strain) were then incubated in anaerobic cabinet for 36 h. Pure culture of each bacterial strain was used as control groups. After incubation, bacterial colonies on plates were counted and inhibitory rate of DM 8504 on periodontal pathogens was calculated. Results The inhibitory rate of DM 8504 on Pg 1312, Pg 33277, Pi 25261, Pi 25611, Aa 29523, Aa Y4 and Fn 10953 were 55 42%, 57 21%, 48 21%, 46 69%, 43 00%, 39 04% and 49 36% respectively. DM 8504 was slightly inhibited in co culture. The inhibitory rate of Pg 33277 on DM 8504 was 21 02%. Statistical analysis showed that difference in viable bacterial enumeration of each strain between pure culture and co culture was highly significant (P<0 01). Conclusion Bifidobacterium DM 8504 has inhibitory effect on periodontal pathogens Pg, Fn, Pi and Aa.

关 键 词：双岐杆菌 吉病 微生物学 抗生作用 集落计数 

分 类 号：R781.4[医药卫生—口腔医学]