酵母来源血管紧张素转移酶抑制六肽性质研究  

Study on properties of Angiotensin-i-converting enzyme inhibitory hexapeptide derived from yeast

作　　者：倪贺[1] 李琳[1] 胡松青[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东省天然产物绿色加工与产品安全重点实验室,广东广州510640

出　　处：《食品工业科技》2012年第17期85-88,共4页Science and Technology of Food Industry

基　　金：中央高校基本科研业务费专项资金资助(2012ZG0007);新世纪优秀人才支持计划资助(NCET-10-0362);973计划资助(2012CB720801)

摘　　要：对具有良好ACE抑制效果的酵母来源ACE抑制六肽(TPTQQS)进行了性质研究。建立了TPTQQS的RP-HPLC的检测方法,并考察了其热稳定性及抗消化性能。结果证实,以含0.05%TFA的超纯水为流动相对TPTQQS进行分析,该方法具有良好的精密度及准确度,能够对TPTQQS进行定性及定量分析,该方法的平均加样回收率为99.08%。在该检测条件下研究该六肽的热稳定性及抗消化性能,结果证实,该多肽在37～60℃下具有良好的热稳定性,并且能够有效抵抗胃肠道蛋白酶的消化。The properties of an ACE inhibitory hexapeptide （TPTQQS） from yeast were investigated. RP HPLC determination method was established. The results showed that TPTQQS could be qualitative and quantitative analysis by RP HPLC, when water contained 0.05% TFA was used as mobile phase. The RP HPLC method showed good precision and accuracy, and the recovery rate of TPTQQS was 99.08%. In this determination condition,thermal stability and antidigestion were researched.The results showed that TPTQQS demonstrated good thermal stability at 3760°C.and could resist diaestion of aastrointestinal tract Droteases.

关 键 词：血管紧张素转移酶抑制六肽 热稳定性 抗消化性 

分 类 号：TS201.2[轻工技术与工程—食品科学]