超声波微波萃取-高效液相色谱法检测肉汤中的罂粟碱  

Determination on papaverine in meat broth by Ultrasonic-microwave synergistic extraction-HPLC

作　　者：信海红[1] 王庭欣[1] 朱路甲[1] 张晓宇[1] 王羽[2] 

机构地区：[1]河北大学质量监督学院,保定071000 [2]河北农业大学食品科技学院,保定071000

出　　处：《中国食品添加剂》2012年第4期250-252,共3页China Food Additives

基　　金：河北省科学技术研究与发展计划;项目编号:114572150

摘　　要：罂粟壳通常作为一种违法添加剂应用于火锅底料,目的是使人吃了上瘾,罂粟壳中含有多种生物碱,溶于肉汤食用后有害于健康。用75%的乙醇作为罂粟碱的提取液,利用超声波微波萃取仪对样品进行提取,高效液相色谱法(HPLC)进行含量分析,建立测定肉汤中罂粟碱的超声波微波萃取-高效液相色谱方法。其结果此方法罂粟碱在0.2 10mg/L范围内呈良好的线性关系,相关系数为0.9999。其平均回收率为94.47%;精密度相对标准偏差(RSD)为0.029%;重现性相对标准偏差(RSD)为0.665%。本方法具有回收率高、灵敏度高、精密度高等优点,可适用于食品中罂粟壳生物碱的检测,为确保农产品餐桌质量安全监控提供技术保障。Pericarpium Papaverisis an illegal additives and usually used as food additives in the hot pot to let people ad- dicted to the dish. Pericarpium Papaveris reacts with many typesof alkaloids in the dish and is harmful to healthA meth- od for the determination of papaverine in meat broth using Ultrasonic - microwave synergistic extraction - HPLC was de- veloped. The Ultrasonic - microwave synergistic extraction was used to extract the sample. In the concentration of 0. 2mg/L tol0mg/L, the result was linear （r =0. 9999）. The average recovery was 94. 47% and the RSD of the pre- cision was 0. 029% and the RSD of the reproducibility was 0. 665%. The method had high recovery, sensitivity and is very precise. It is suitable for the determination of papaverine in food. It provides the technical support to monitor the safety of agricultural products.

关 键 词：超声-微波协同萃取 液相色谱 罂粟碱 检测方法 

分 类 号：TS207.3[轻工技术与工程—食品科学]