茯苓总三萜对小鼠肝损伤的预防作用  

Preventive Effect of Triterpenes from Poria cocos on Liver Injury in Mice

作　　者：张先淑[1] 饶志刚 胡先明[2] 刘鹏 

机构地区：[1]重庆工贸职业技术学院,重庆408000 [2]病毒学国家重点实验室,组合生物合成与新药发现教育部重点实验室,武汉大学药学院,湖北武汉430071

出　　处：《食品科学》2012年第15期270-273,共4页Food Science

基　　金：国家科技部重大专项(2009ZX09301-14-1)

摘　　要：目的:研究茯苓三萜对四氯化碳(CCl4)所致小鼠肝损伤的治疗作用。方法:将小鼠随机分为正常组、模型组、联苯双酯组及茯苓三萜高、中、低剂量组共6组。小鼠腹腔注射CCl4橄榄油溶液造模。治疗组以高、中、低剂量(180、90、45mg/(kg.d))茯苓三萜进行灌胃,阳性药物对照组用联苯双酯滴丸(180mg/(kg.d)),皆以1g/100mL羧甲基纤维素钠溶液混悬,0.2mL/10g体质量进行灌胃;正常组与模型组以0.9%生理盐水(0.2mL/10g)体质量灌胃。最后摘眼球取血,收集血清,检测丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)活性;以及小鼠肝组织HE染色后做病理切片观察。结果:与模型组相比茯苓三萜治疗组能够显著降低小鼠血清中AST、ALT活性。病理切片清楚地显示茯苓三萜能够显著减轻小鼠肝损伤的程度。结论:茯苓三萜对CCl4所致的小鼠肝损伤有明显治疗作用。Objective ：To explore the preventive effect of triterpenes from Poria cocos on carbon tetrachloride-induced liver injury in mice.Methods： Kunming mice were randomly divided into normal group,model group,（dimethyl diphenyl bicarboxylate,DDB） positive control group,and low-,middle-and high-dose treatment groups.Mouse models of liver injury were created by intraperitoneal injection of CCl4.The high-,middle-and low-dose treatment groups were orally administered with triterpenes from Poria cocos at doses of 180,90 mg/（kg.d） and 45 mg/（kg.d）,respectively.The positive control group was orally administered with DDB at a dose of 180 mg/（kg.d）.Both drugs were separately suspended in 1 g/100 mL CMC-Na solution before administration and given at a level of 0.2 mL/10 g body weight.The normal and model groups were given 0.9% normal saline at a level of 0.2 mL/10 g body weight.The serum AST and ALT activities were determined and liver injury was evaluated by HE staining.Results： Compared with the model group,the three treatment groups showed significantly lower AST and ALT activities.In addition,Poria cocos triterpenes could significantly attenuate liver injury in mice.Conclusion： Poria cocos triterpenes have obvious protective effect on CCl4-induced liver injury in mice.

关 键 词：茯苓三萜 小鼠 肝损伤 四氯化碳 治疗作用 

分 类 号：TS201.21[轻工技术与工程—食品科学]