微生物发酵爆破秸秆生产动物饲料培养基的优化  

Optimization of Exploded Corn Stover Substrate by Microbial Fermentation for Animal Feed Production

作　　者：常娟[1] 尹清强[1,2] 郑秋红[1] 姜义宝[1] 郭红伟[1] 左瑞雨[1] 刘俊熙 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南省饲料微生物工程技术研究中心,河南周口466000

出　　处：《河南农业科学》2012年第7期142-145,共4页Journal of Henan Agricultural Sciences

基　　金：郑州市科技创新团队项目(112PCXTD339)

摘　　要：为了促进秸秆饲料资源开发及其在动物饲养中的应用,对爆破玉米秸秆米曲霉固体发酵生产动物饲料的固体发酵培养基进行优化。以爆破玉米秸秆为主要原料(81%～100%),以玉米(0、2.5%、5.0%)、麸皮(0、2.5%、5.0%)、豆粕(0、2.5%、5.0%)和营养液(0、2.0%、4.0%)设计四因素三水平正交试验,固体发酵6d后,测定发酵产物纤维素降解率、滤纸酶活性和羧甲基纤维素(CMC)酶活性,选择最佳发酵培养基。结果表明,纤维素降解率和滤纸酶活性最高的培养基组合为:83.5%爆破玉米秸秆、5.0%玉米、2.5%麸皮、5.0%豆粕和4.0%营养液,其中营养液、豆粕和玉米的不同水平对纤维素的降解率有显著影响(P<0.05)。CMC酶活性最高的培养基组合为:86%爆破玉米秸秆、2.5%玉米、2.5%麸皮、5.0%豆粕和4.0%营养液。综合结果表明,爆破秸秆发酵生产动物饲料的最优培养基组合为:83.5%爆破秸秆、5.0%玉米、2.5%麸皮、5.0%豆粕和4.0%营养液。The solid fermentation substrate of exploded corn stover fermented by Aspergillus oryzae for producing animal feed was optimized.The four-factor and three-level orthogonal expe-riment of exploded corn stover（81%-100%）,corn meal（0%,2.5% and 5.0%）,wheat bran（0%,2.5% and 5.0%）,soybean meal（0%,2.5% and 5.0%） and nutrient solution（0%,2.0% and 4.0%） was used in this experiment,and the culture medium was optimized by determining the cellulose degradation,the filter paper cellulose enzyme activity（FPA）and CMCase activity of the fermented product.The result showed that the optimal medium for biodegradation and FPA production of exploded corn stover was 5% corn meal,2.5% wheat bran,5.0% soybean meal and 4.0% nutrient solution.The levels of nutrient solution,corn meal,and soybean meal had significant effects on stover degradation（P0.05）.The optimal medium for CMCase production of exploded corn stover was 2.5% corn meal,2.5% wheat bran,5.0% soybean meal and 4.0% nutrient solution.This work obtained the best culture medium for producing biological corn stover feed was 83.5% exploded corn stover,5.0% corn meal,2.5% wheat bran,5.0% soybean meal and 4.0% nutrient solution.

关 键 词：爆破秸秆 米曲霉 固体发酵 正交试验 

分 类 号：S816.5[农业科学—饲料科学]