犬瘟热病毒GZ2株的分离鉴定及H基因原核表达质粒构建  

Isolation and Identification of the Canine Distemper Virus Guizhou Strain (CDV GZ2) and Prokaryotic Expression Plasmid Construction of the Viral Hemagglutinin Gene

作　　者：曾智勇[1,2] 周莉[1] 汤德元[1] 刘志杰[1] 梁海英[3] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病研究室,贵州贵阳550025 [3]贵州大学教学实验场,贵州贵阳550025

出　　处：《贵州农业科学》2012年第8期165-168,共4页Guizhou Agricultural Sciences

基　　金：贵州大学博士基金"犬瘟热病毒贵州株的分离鉴定及主要抗原蛋白编码基因的研究"(X060054)

摘　　要：对疑似CD的贵宾犬病料进行了CDV的分离和初步鉴定以及H基因的克隆和序列分析,以探讨不同分离株之间H基因的差异。结果表明:采用同步接种的方法,分离到1株犬瘟热病毒(CDV GZ2),并以其RNA为模板,扩增克隆了该病毒血凝素(H)基因,进而构建原核表达质粒pET-H。该毒株H基因ORF大小为1 824bp,可编码607个氨基酸;与国内分离株氨基酸一致性为97.4%～98.5%,与国外分离株为89.6%～95.6%,与疫苗株在89.6%～91.6%。进化分析表明:CDV的分布具有一定地域性,CDV GZ2与国内东北地区分离株亲缘关系近,而与国外分离株亲缘关系相对较远。The canine distemper virus（CDV GZ2） was isolated from suspicious CD pathologic material（poodle） and preliminarily identified,then the viral H gene was cloned and the sequence was analyzed to explore the difference of H gene among different isolated strains.The results showed that a CDV GZ2 strain was isolated by synchronization inoculation,and the viral H gene was cloned taking the viral RNA as templates,thus the prokaryotic expression plasmid was constructed.The viral H gene ORF was 1 824 bp,encoding 607 amino acids,97.4%～98.5% consistent with the strains in China,but 89.6%～95.6% with the strains abroad,and 89.6%～91.6% with vaccine strain.CDV GZ2 and the strains isolated from northeast area in China had the same ancestor,but the genetic relationships between CDV GZ2 and the strains from abroad were far.

关 键 词：犬瘟热病毒 分离鉴定 H基因 原核表达 质粒构建 

分 类 号：S852.655[农业科学—基础兽医学]