竞争性抑制酶联反应在量热式农残生物传感器中的试验研究  

Research of thermal pesticide biosensor in the method of competitive inhibition of enzyme reactions

作　　者：Nguyen Van-luc 于劲松[1] 徐斐[1] 华泽钊[1] 

机构地区：[1]上海理工大学食品安全研究所,上海200093

出　　处：《东北农业大学学报》2012年第8期25-29,共5页Journal of Northeast Agricultural University

基　　金：国家自然基金(31101283);上海市科委重点项目(10391901500);上海市科技兴农重点攻关项目(2006第7-4号)

摘　　要：以量热式农残生物传感器为研究对象,借鉴免疫分析方法,进行竞争性抑制酶联反应在量热式农残生物传感器中的试验研究。结果表明,在甲胺磷浓度为0~0.004 mg.kg-1范围内,甲胺磷含量和放热量之间的线性较好,竞争性抑制酶联反应分析法测农药残留的最低检测限为0.0011 mg.kg-1,每次测定时间为20 min。Drawing on immunoassay methods,the preliminary test of thermal biosensor was researched in the method of competitive inhibition of enzyme reactions.The results showed that,at concentration of 0-0.004 mg.kg-1 of methamidophos,there is good linearity between methamidophos content and heat release.The limit of detection of competitive inhibition of enzyme reactions method is 0.0011 mg.kg-1,and the measurement time is about 20 minutes.

关 键 词：酶标农药 鸡肝酯酶 量热式酶传感器 竞争性抑制酶联反应 

分 类 号：TS201.6[轻工技术与工程—食品科学]