一株毛栓菌静置液体发酵产纤维素酶条件研究  

Static liquid fermentative conditions for producing cellulase of Trametes trogii

作　　者：贾翠英[1] 张玉辉[1] 王振河[1] 

机构地区：[1]河南科技学院生命科技学院,河南新乡453003

出　　处：《菌物学报》2012年第5期736-744,共9页Mycosystema

基　　金：河南省科技攻关项目(No.112102310443)

摘　　要：考察一株新分离的毛栓菌静置产纤维素酶的条件,通过单因素试验和正交设计试验分别对培养基初始pH、培养基中的碳源、氮源及诱导物进行研究。利用DNS(3,5-二硝基水杨酸)法测定CMC-Na(羧甲基纤维素钠)纤维素酶活。结果表明:该毛栓菌静置发酵产纤维素酶的最适pH为5.0,最适碳源、氮源和诱导物分别是淀粉、酵母粉和干麸皮,且质量浓度分别为淀粉1%、酵母粉0.4%、干麸皮1.5%,在此条件下,进行静置发酵3d,可获得CMC-Na(羧甲基纤维素钠)纤维素酶活力最高为56.62U/mL。此研究开创了纤维素酶生产菌的新发现,与以往所报道的好氧产纤维素酶菌种黑曲霉、木霉等相比,具有发酵时间短、耗能小等优点,具有一定的潜在应用价值。In order to investigate the influence of the factors such as the initial pH of fermentative medium, the kinds of carbon and nitrogen sources of fermentative medium and the types of inducers on CMC-Na （monosodium carboxymethylcellulose） cellulase activity of a new isolated producing cellulase strain from the rumen of sheep, the single factor and orthogonal test were respectively carried out. The CMC-Na cellulase activity was determined by DNS （3,5-dinitryl salicylate） method. The results displayed that the optimal initial pH value of fermentative medium was 5.0. The optimal carbon and nitrogen sources of fermentative medium were respectively starch and yeast extract and their optimal additive amount were respectively 1% and 0.4%. The optimal inducer was dry bran （1.5%）. Under the optimal condition, the highest value of CMC-Na cellulase activity was 56.62U/mL. All these results indicated that this was a new founding for looking for strain producing cellulase and using Trametes trogii to produce cellulase by static fermentation showed some advantages such as short fermentation time, and lower energy cost as compared with the reported Aspergillus niger and Trichoderma. Therefore, this study will display some certain substantial economic significance.

关 键 词：毛栓菌 纤维素酶 静置发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]