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机构地区:[1]宁波大学食品科学与工程系,浙江宁波315211
出 处:《中国食品学报》2012年第8期53-59,共7页Journal of Chinese Institute Of Food Science and Technology
基 金:国家农业科技成果转化基金项目(2009GB2C220412);国家自然科学基金项目(30972130);浙江省自然科学基金重大项目(Z3110211);浙江省自然科学基金(LQ12C20003);浙江省重大科技专项重点农业项目(2010C12015);宁波市农业创新创业资金重点项目(2010C92024);宁波市重大攻关项目(2011C11017)
摘 要:以分离自新疆酸马奶,具有高效降胆固醇活性的发酵乳杆菌SM-7和1株乳酸乳球菌乳亚种LB12菌株发酵制备酸奶,通过单因素试验研究混菌比例、接种量、发酵时间和发酵温度对酸奶品质的影响。通过结合感官评定为指标的正交试验,确定酸奶最佳发酵条件是:菌种配比1∶1(发酵乳杆菌:乳酸乳球菌),接种量6%,发酵温度42℃,发酵时间8h。利用该条件发酵的酸奶,组织状态较粘稠,乳清析出少,香气较好,酸甜适宜。其胆固醇降解率达44.86%,比优化前提高了4.29%。In this study, Lactobacillus fermentum SM-7 and Lactococus. lactis subsp. lactis LB12 with cholesterol-reducing function were used to ferment yogurt. The Lactobacillus fermentum strain SM-7 with cholesterol-lowering effects was isolated from koumiss in Xingjiang Province. Lactococus. lactis subsp. lactis LB12 preserved in our laboratory was also with cholesterol-reducing function. The effects of strain ratio, inoculum amount, fermentation time and fermentation temperature were investigated to evaluate yogurt sensory quality. The optimal fermentation condition was obtained through orthogonal test: Lactobacillus fermentum SM-7: Lactococcus.lactis subsp. lactis LB12 of 1∶1, inoculum amount of 6% and fermentation at 42 ℃ for 8 h. The yogurt was with good sensory quality and had cholesterol-degradation rate of 44.86% which increased by 4.29% than before optimization.
关 键 词:发酵乳杆菌SM-7 乳酸乳球菌乳亚种LB12 酸奶 降胆固醇 感官品质
分 类 号:TS252.54[轻工技术与工程—农产品加工及贮藏工程]
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