低能N离子注入对蚕豆根尖细胞的诱变效应  

Effects of Irradiation with Low-energy Nitrogen Ion Injection on Root Tip Cells of Broad Bean

作　　者：黄雅琴[1] 李尽哲[1] 黄群策[2] 

机构地区：[1]信阳农业高等专科学校,河南信阳464000 [2]郑州大学离子束生物工程省重点实验室,河南郑州450052

出　　处：《原子核物理评论》2012年第3期290-293,共4页Nuclear Physics Review

基　　金：河南省科技前沿基础研究项目(82300433202)~~

摘　　要：为了研究低能N离子束的细胞遗传学效应,以不同剂量的N离子束对蚕豆种子的种胚进行辐照,观察分析根尖细胞的微核率、有丝分裂指数和染色体畸变效应。研究发现,离子束的注入抑制了根尖细胞的有丝分裂,干扰了正常的有丝分裂过程,引发了染色体的结构、行为和数目畸变;随着离子注入剂量的增加,微核率增加、有丝分裂指数降低、染色体畸变率增加。In order to study the cytogenetic effects of low-energy nitrogen ion irradiation, broad bean seed embryo was irradiated by different doses of nitrogen ions. Micronucleus rate, mitotic index and chromo- some aberration in root-tip cells were analyzed. The results showed that the injection of ions inhibited mi- tosis of root tip cells, interfered the normal process of mitosis, caused aberrations of chromosome struc- ture, behavior and number. The frequency of micronucleus and chromosomal aberrations increased with the increasing radiation dosage, while mitotic index decreased.

关 键 词：N离子束 蚕豆 根尖细胞 染色体畸变 微核 

分 类 号：Q691[生物学—生物物理学]