Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse  

作　　者：Qiuling Li Qiongxiang Zhai Jia Geng Hui Zheng Fei Chen Jie Kong Cheng Zhang 

机构地区：[1]Department of Neurology, FirstAffiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China [2]Department of Pediatrics, Guangdong General Hospital Guangdong Neuroscience Institute, Guangdong Academy of Medical Sciences Guangzhou 510080, Guangdong Province, China [3]Department of Neurology, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China [4]Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China [5]Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou 510085, Guangdong Province, China

出　　处：《Neural Regeneration Research》2012年第22期1695-1702,共8页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.30370510,30170337,30400322,30870851;CMB Fund,No.4209347;Key Project of the State Ministry of Public Health,No.2001321;Fok Ying Tung Education Foundation,No.91029;Key Projects in the National Science and Technology Pillar Program During the Eleventh Five-Year Plan Period,No.2006BAI05A07

摘　　要：In this study, recombinant baculovirus carrying the microdystrophin and β-catenin genes was used to infect adipose-derived stem cells from a dystrophin-utrophin double knock-out mouse. Results showed that, after baculovirus transgene infection, microdystrophin and β-catenin genes were effectively expressed in adipose-derived stem cells from the dystrophin-utrophin double knock-out mouse. Furthermore, this transgenic expression promoted adipose-derived stem cell differentiation into muscle cells, but inhibited adipogenic differentiation. In addition, protein expression related to the microdystrophin and Wnt/β-catenin signaling pathway was upregulated. Our experimental findings indicate that baculovirus can successfully deliver the microdystrophin and β-catenin genes into adipose-derived stem cells, and the microdystrophin and Wnt/β-catenin signaling pathway plays an important role in myogenesis of adipose-derived stem cells in the dystrophin-utrophin double knock-out mouse.In this study, recombinant baculovirus carrying the microdystrophin and β-catenin genes was used to infect adipose-derived stem cells from a dystrophin-utrophin double knock-out mouse. Results showed that, after baculovirus transgene infection, microdystrophin and β-catenin genes were effectively expressed in adipose-derived stem cells from the dystrophin-utrophin double knock-out mouse. Furthermore, this transgenic expression promoted adipose-derived stem cell differentiation into muscle cells, but inhibited adipogenic differentiation. In addition, protein expression related to the microdystrophin and Wnt/β-catenin signaling pathway was upregulated. Our experimental findings indicate that baculovirus can successfully deliver the microdystrophin and β-catenin genes into adipose-derived stem cells, and the microdystrophin and Wnt/β-catenin signaling pathway plays an important role in myogenesis of adipose-derived stem cells in the dystrophin-utrophin double knock-out mouse.

关 键 词：BACULOVIRUS adipose-derived stem cells Duchenne muscular dystrophy microdystrophin β-catenin MYOGENESIS gene therapy neural regeneration 

分 类 号：Q811.4[生物学—生物工程] TQ453.5[化学工程—农药化工]