宫颈癌与微卫星不稳定性的相关性研究  

Relationship of microsatellite instability with cervical carcinoma

作　　者：王芳[1] 平智广[2] 袁佩[1] 

机构地区：[1]南京医科大学附属常州市妇幼保健院妇产科,常州213003 [2]郑州大学公共卫生学院卫生统计学教研室,郑州450001

出　　处：《第二军医大学学报》2012年第9期991-995,共5页Academic Journal of Second Military Medical University

摘　　要：目的研究宫颈癌与微卫星不稳定性的相关性,为探索宫颈癌的发病风险提供新的研究途径。方法采用PCR结合毛细管电泳的方法对常州地区120例宫颈癌患者(病例组)和102例健康女性(对照组)的外周静脉血样进行D1S1627、D22S1045基因座的基因型分析,并根据两组在D1S1627、D22S1045基因座的等位基因分布差异来推测与宫颈癌发生相关的易感因素和(或)抗性因素。结果病例组和对照组在D22S1045基因座上的等位基因频率分布差异存在统计学意义(P=0.008),在D1S1627基因座上的等位基因频率分布差异无统计学意义(P=0.095)。单因素分析显示两组在D22S1045基因座的等位基因14、17之间的频率分布差异均有统计学意义(P=0.026,P=0.014),OR值分别为3.837(95%CI:1.078～13.658)、0.520(95%CI:0.306～0.882)。多变量logistic回归分析显示产次、吸烟、D22S1045基因座等位基因14均为与宫颈癌发生相关的危险因素,OR值分别为2.063(95%CI:1.070～3.975)、1.958(95%CI:1.002～3.826)、2.766(95%CI:1.039～7.360)。结论 D22S1045基因座可能与宫颈癌的发生相关联,该基因座的等位基因14为宫颈癌的易感因素,等位基因17为宫颈癌的抗性因素。Objective To investigate the relationship of cervical carcinoma with microsatellite instability, so as to provide a new pathway for investigating the risks of cervical carcinoma. Methods PCR and electrophoresis method were used to conduct genotype analysis on D1S1627 and D22S1045 in the peripheral blood of 120 cervical carcinoma patients and 102 healthy women living in Changzhou area. The susceptible and（or） resistant factors of cervical carcinoma were evaluated based on the differences in allele distribution of D1S1627 and D22S1045. Results There were significant differences in locus D22S1045 （P = 0. 05） between cervical carcinoma group and healthy group, not on locus D1S1627 （P=0. 995）. Univariate analysis showed significant differences at allele 14 and 17 on locus D22S1045 between the two groups （P=0. 026, P=0. 014）, with the OR values being 3. 837 （95 % CI： 1. 078-13. 658） and 0. 520 （ 95% CI ：0. 306-0. 882）, respectively. Multivariate logistic regression analysis results showed that parity, smoking and allele 14 of D22S1045 were the risk factors of cervical carcinoma, with the OR value being 2. 063（95%CI：1. 070-3. 975） ,1. 958（95%Ch 1. 002-3. 826） and 2. 766（95%CI： 1. 039-7. 360）, respectively. Conclusion Locus D22S1045 might be associated with cervical carcinoma, with allele 14 being the susceptible factor and cellele 17 being the resistant factor.

关 键 词：宫颈肿瘤 微卫星不稳定性 疾病遗传易感性 

分 类 号：R737.33[医药卫生—肿瘤]