茶多酚中儿茶素类的HPLC分析  

HPLC Analysis of Catechins in Tea Polyphenols

作　　者：刘小乔[1] 李忠岐[1] 高静[1] 陈蓓[1] 杨建雄[1] 

机构地区：[1]陕西师范大学生命科学学院,西安市710062

出　　处：《光谱实验室》2012年第5期2611-2615,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金资助项目(20175012)

摘　　要：建立一种用高效液相色谱法(HPLC)测定茶叶中儿茶素类的方法,建立了同时分离测定了5种儿茶素组分(EGC、C、EGCG、EC、ECG)及咖啡碱(CAF)的方法,得到的最佳色谱条件为:ODS(5μm,4.6mm×250mm)色谱柱,流动相A:0.1%的磷酸甲醇溶液;流动相B:0.1%的磷酸水溶液;流速为1.0mL/min;柱温为30℃;UV检测波长278nm。儿茶素单体及咖啡碱在给定的浓度范围内线性关系良好,方法精密度和重复性的相对标准偏差在5%以内,测定样品中各标准品的平均加标回收率为98.20%—100.64%。与国标法GB/T 8313-2008相比,本方法解决了国标法中EC和EGCG难分离的问题。An analytical method for the simultaneous qualitative and quantitative analysis of 5 kind of catechins（including EGC,C,EGCG,EC and ECG） and caffeine（CAF） was established by high performance liquid chromatography（HPLC）,and the optimal chromatographic conditions were as follows：ODS column（5μm,4.6mm×250mm）,mobile phase A of 0.1% phosphoric acid in methanol（V/V）,mobile phase B of 0.1% phosphoric acid in water（V/V）,the flow rate of 1mL/min,the column temperature of 30℃ and the detection wavelength of 278nm.The linear relationship of catechins and caffeine was good in a given range of concentration,the RSDs of the method repeatability and the precision were less than 5%,and the average standard recoveries of each standard sample were in the range of 98.20%—100.64% for catechins and caffeine.Meanwhile,compared with the national standard method GB/T 8313-2008,the method can solve the difficult questions of separation of EGCG and EC in national standard method.

关 键 词：儿茶素类 咖啡碱 高效液相色谱法 国标法 

分 类 号：O657.72[理学—分析化学]