高效液相色谱法测定枸杞叶粗蛋白中的活性多肽IN-5  

Determination of IN-5 in Lycium chinense Leaves Crude Protein by HPLC

作　　者：江磊[1,2] 毕洪涛[1] 梅丽娟[1] 王启兰[1] 邵赟[1] 陶燕铎[1] 

机构地区：[1]中国科学院西北高原生物研究所藏药中心,西宁市新宁路23号810008 [2]中国科学院研究生院,北京市100049

出　　处：《光谱实验室》2012年第5期2857-2860,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：科技部国家科技支撑计划(2007BA145B00)

摘　　要：建立高效液相色谱法测定枸杞叶粗蛋白中活性多肽IN-5含量的方法。色谱条件为:色谱柱Kinetex C18(250mm×4.6mm,2.6μm);流动相为乙腈-0.2%磷酸水(5∶95,V/V);检测波长210nm;流速为0.7mL/min;柱温25℃。结果表明,IN-5浓度在16.0—240.0μg/mL内与峰面积的线性关系良好(r=0.9999)。平均回收率99.2%,RSD=0.49%(n=9)。方法快速、准确、重复性好,可用于IN-5含量的检测。The method for determination of the content of active hypoglycemic peptide IN-5 in the crude protein extract of Lycium chinense leaves was established by HPLC.The chromatographic conditions were as follows：Kinetex C18 column（250mm×4.6mm,2.6μm）,mobile phase of acetonitrile-0.2% phosphoric acid（5∶95,V/V）,detection wavelength of 210nm,flow rate of 0.7mL/min and column temperature of 25℃.The results showed that the concentration of IN-5 had a good linear relationship with peak area（r=0.9999） between 16.0μg/mL and 240.0μg/mL.The average recovery was 99.2% with RSD of 0.49%（n=9）.The method is fast,accurate with good repeatability,and can be applied to detection of the content of IN-5.

关 键 词：活性多肽 降血糖 枸杞叶 蛋白提取物 

分 类 号：O657.72[理学—分析化学]