牛磺酸防止白内障形成的组织化学研究  

A histochemical study on the prevention of cataract with taurine

作　　者：闫洪禄[1] 王王秀 

机构地区：[1]青岛市立医院眼科,266011

出　　处：《山东医大基础医学院学报》2000年第2期65-67,共3页

摘　　要：研究牛磺酸对晶体氧化损伤的保护作用。方法 :将正常兔晶体分为正常条件培养组 (对照组 ) ,Feton模型组 (氧化损伤组 ) ,氧化损伤同时给 0 .5%和 1 %牛磺酸保护组。体外培养 2 4 h后 ,检测各组晶体的总抗氧化能力 (T- AOC)水平 ,可溶性蛋白 (sol- prot)含量 ,超氧化歧化酶 (SOD) ,过氧化氢酶 (CAT)及谷胱苷肽过氧化物酶 (GSH- PX)活性 ,以及脂质过氧化反应产物丙二醛 (MDA)含量。结果 :和对照组比较 ,Feton模型组晶体 T- AOC水平 ,SOD、CAT和 GSH- PX活性 ,slo- prot含量明显降低 ,MDA含量明显升高 ;和 Feton模型组相比 ,牛磺酸保护组晶体 T- AOC、SOD、CAT、GSH- PX、sol- prot明显升高 ,MDA明显下降 ,以 1 %牛磺酸组最为明显。结论 :牛磺酸有保护体外培养的兔晶体免受氧化损伤 ,抑制其脂质过氧化的作用。Objective:To evaluate the effect of taurine on protecting lens from oxidative stress.Methods:Rabbits′ lenses cultured in vitro were divided into 4 groups:control group,Feton model(oxidative stress group),the Feton model treated with 0.5% taurine and the Feton model with 1% taurine.The levels of lens′ T AOC,sol prot,SOD,CAT,GSH PX and MDA were tested after incubation for 24h.Results:The levels of lens′ T AOC,sol prot,SOD,CAT,GSH PX were significantly decreased in Feton model group compared with control group,and were significantly increased in the groups treated with 0.5% and 1% taurine compared with Feton model group,and were significantly increased in 1% taurine group compared with 0.5% taurine group.The level of lens′ MDA was significantly increased in Feton model group compared with control group,and markedly decreased in 0.5% and 1% taurine groups,especially in the 1% taurine group.Conclusion:Taurine can protect lens from oxidative stress in vitro.

关 键 词：牛磺酸 晶体 白内障 组织化学 

分 类 号：R776.102[医药卫生—眼科]