HPLC法测定阜康阿魏根和叶中阿魏酸的含量  

Determination of Ferulic acid content in roots and leaves of Ferula fukanensis K.M. Shen. by HPLC

作　　者：王东东[1] 刘宏炳[1] 宋海龙 杨卫星[3] 贾晓光 田树革[1] 

机构地区：[1]新疆医科大学中医学院,乌鲁木齐830011 [2]新疆维吾尔自治区中药民族药研究所,乌鲁木齐830002 [3]新疆昌吉卫生学校,新疆昌吉831100

出　　处：《新疆医科大学学报》2012年第9期1139-1142,共4页Journal of Xinjiang Medical University

基　　金：第四次全国中药资源普查(试点)新疆中药民族药资源普查专项资助(2011-2013)

摘　　要：目的建立阜康阿魏根和叶中阿魏酸含量的测定方法。方法采用高效液相色谱法(HPLC)测定阜康阿魏根和叶中阿魏酸的含量。色谱条件:Kromasil C18柱(4.6mm×150mm,5μm),流动相为乙腈-0.4%磷酸(15∶85),流速为1.0mL/min,检测波长为320nm,柱温为35℃。结果阿魏酸峰面积与进样量在0.009 8～0.049 0μg范围内有良好的线性关系(r=0.999 6),平均回收率为99.3%,RSD为0.79%(n=6)。结论该方法快速、准确、可靠,可用于阿魏根和叶中阿魏酸含量的测定。Objective To establish a method for the content determination of ferulic acid in roots and leaves of Ferula fukanensis K. M. Shen.. Methods The content of ferulic acid was detected by HPLC. The sep- aration was carried out on a Kromasil C18 column （150 mm×4.6 mm, 5 fire）. The mobile phase was ace- tonitrile-0.4% phosphoric acid solution（15 ： 85）; the flow rate was 1.0 mL/min; the detective wave- length was set at 320 nm. The column temperature was 35℃. Results The calibration curve showed good linearity over the range of 0. 009 8-0. 049 0 μg （r =0. 999 6）. The average recovery was 99.29 % with RSD 0.79% （n=6）. Conclusion This method is rapid, accurate, reliable; it could be used as a determi- nation of ferulic acid in root and leaf of Ferula fukanensis K.M. Shen..

关 键 词：阜康阿魏 阿魏酸 高效液相色谱法 含量测定 

分 类 号：R914[医药卫生—药物化学]