Determination of Vitamins B2,B3,B6 and B7 in Corn Steep Liquor by NIR and PLSR  

作　　者：肖雪 侯媛媛 杜军 孙丹 白钢 罗国安 

机构地区：[1]College of Pharmacy,Nankai University [2]Department of Chemistry,Tsinghua University [3]College of Life Science,Nankai University [4]China Biotech Fermentation Industry Association

出　　处：《Transactions of Tianjin University》2012年第5期372-377,共6页天津大学学报（英文版）

基　　金：Supported by Foundation of Tianjin City Science and Technology Project (No.09ZCKFSH00900)

摘　　要：Corn steep liquor(CSL) is an important raw material that has high nutritional value and serves as a nitrogen source.Biotin in CSL is especially of great importance to fermentation.In order to develop a fast,versatile,cheap,and environmentally safe analytical method for quantifying vitamins B2(VB2),B3(VB3),B6(VB6) and B7(VB7) in CSL,the near-infrared spectroscopy(NIR) measurements of 66 samples(22 batches) of CSL were analyzed by partial least-square regression(PLSR).Multivariate models developed in the NIR regions showed good predictive abilities for VB2,VB3,VB6 and VB7.Results confirmed the probability of the multivariate spectroscopic approach as a replacement for expensive and time-consuming conventional chemical methods.Corn steep liquor （CSL） is an important raw material that has high nutritional value and serves as a nitro- gen source. Biotin in CSL is especially of great importance to fermentation. In order to develop a fast, versatile, cheap, and environmentally safe analytical method for quantifying vitamins B2 （VB2）, B3 （VB3）, B6 （VB6） and B7 （VB7） in CSL, the near-infrared spectroscopy （NIR） measurements of 66 samples （22 batches） of CSL were ana- lyzed by partial least-square regression （PLSR）. Multivariate models developed in the NIR regions showed good pre- dictive abilities for VB2, VB3, VB6 and VB7. Results confirmed the probability of the multivariate spectroscopic ap- proach as a replacement for expensive and time-consuming conventional chemical methods.

关 键 词：corn steep liquor NIR RIBOFLAVIN nicotinic acid PYRIDOXINE BIOTIN multivariate calibration PLSR 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]