氧化三酰甘油聚合物的细胞毒性研究  

Toxicity of oxidized triglyceride polymers in Ana-1 cells

作　　者：曹文明[1,2] 曹虹晖[3] 薛斌 陈凤香 袁风娇[4] 施恒飞[4] 张伟云[4] 

机构地区：[1]江南大学食品学院,无锡214122 [2]上海市粮食科学研究所,上海200136 [3]中国药科大学药学院,南京211198 [4]南京大学医学院,南京210093

出　　处：《食品科技》2012年第9期171-174,179,共5页Food Science and Technology

基　　金：国家十二五科技支撑项目(2012BAK08B03);国家自然基金面上项目(31171703);上海市科委应用技术开发专项(2010-115);上海市科委科研计划项目(11142200400,11231200100)

摘　　要：食用植物油经深度氧化劣败会产生高浓度的氧化三酰甘油聚合物(TGP),TGP是氧化三酰甘油(oxidized triacylglycerol,ox-TG)的二聚体(oxidized triacylglycerol dimers,TGD)和寡聚体(oxidized triacylglycerol oligomers,TGO)的总称。研究旨在初步评价TGD和TGO的生物学毒性。以不同浓度的TGP作用于体外培养的巨噬细胞,分别检测细胞的增殖能力、中性红吞噬能力和LDH释放量,同时采用激光共聚焦显微镜观察细胞凋亡、流式细胞术检测细胞内ROS水平。结果显示,TGP显著抑制巨噬细胞的增殖能力和中性红吞噬能力,显著提高细胞内ROS水平和LDH释放量,导致巨噬细胞的凋亡。因此,初步证实TGP具有明显的生物学毒性,对机体的免疫功能有损伤作用。Edible vegetable oil usually produces high concentrations ofoxidized triglyceride polymers （TGP） after deeply oxidative deterioration. The aim of the present study is to evaluate the toxicity of TGP. Cultured macrophages were treated with different concentrations of TGP and then the cell proliferation ability, the phagocytic activity and LDH release were detected. Besides, apoptosis were observed by laser scanning confocal microscopy, and ROS were determined by flow cytometry. The results showed that proliferation ability and phagocytic activity of macrophages were significantly inhibited by TGP. TGP also significantly increased the ROS level and LDH release of cells, leading apoptosis in macrophages. Therefore, it is suggested that TGP has obvious toxicity and may damage the immune function.

关 键 词：氧化三酰甘油聚合物 巨噬细胞 毒性 

分 类 号：TS201.4[轻工技术与工程—食品科学]