油茶皂素大孔树脂纯化工艺优化及LC/TOF-MS分析  

Purification by Macroporous Adsorption Resin Chromatography and LC/TOF-MS Analysis of Sasanquasaponin

作　　者：张新富[1,2] 朱翔[3] 刘亚军[3] 夏涛[1] 杜先锋[1] 高丽萍[3] 张德龙[4] 

机构地区：[1]安徽农业大学茶叶生物化学与生物技术教育部农业部重点实验室,安徽合肥230036 [2]青岛农业大学园林园艺学院,山东青岛266109 [3]安徽农业大学生命科学学院,安徽合肥230036 [4]安徽农业大学微生物防治安徽省重点实验室,安徽合肥230036

出　　处：《食品科学》2012年第16期7-11,共5页Food Science

基　　金："十一五"国家科技支撑计划项目(2009BADB1B10)

摘　　要：以市售低纯度茶皂素粗品为原料,采用AB-8大孔树脂进行纯化,得到最佳纯化工艺为30g/L粗皂素溶液上样,30%乙醇洗脱除去多数色素和部分蛋白等杂质,70%乙醇溶液主要洗脱出茶皂素,油茶皂素的得率为5 5.5%,纯度提高1.8 6倍。大孔树脂重复利用8次后,吸附和洗脱效果基本不变,放大试验证明其适合工业化生产。LC/TOF-MS分析表明油茶皂素的分子质量主要集中在1170～1304D之间,并确定了一种油茶皂素的分子式为C5 8H8 9O2 6,同时推测了其结构式。AB-8 macroporous resin was used to purify commercial crude sasanquasaponin with low purity. The optimal purification process was determined as follows： 30 g/L crude sample solution was loaded followed by washing the resin with 30% ethanol aqueous solution to remove unwanted impurities including most pigments and partial proteins, and the adsorbed sasanquasaponin was finally eluted with 70% ethanol aqueous solution, resulting in a recovery of 55.5% and a purification factor of 1.86. After eighth repeated use, the adsorption and elution capacities of this resin were unchanged basically. Furthermore, the scale-up experiments demonstrated the suitability for industrial production. The LC-TOF-MS analysis indicated that the molecular weight of the purified sasanquasaponin mainly varied between 1170 and 1304 D. In addition, a sasanquasaponin compound with molecular formula C58H89O26 was identified and its structural formula was deduced.

关 键 词：茶皂素 大孔树脂 LC/TOF-MS 

分 类 号：R284.2[医药卫生—中药学]