北五味子种子和果肉中的活性成分比较  

Comparative Analysis of Bioactive Component in Seeds and Flesh of Schisandrae chinensis Fructus by Thin Layer Chromatography

作　　者：李立顺[1] 时维静[1] 马迅兵[1] 

机构地区：[1]安徽科技学院食品药品学院,安徽凤阳233100

出　　处：《食品科学》2012年第16期175-178,共4页Food Science

基　　金：安徽省科技厅科技攻关重点项目(07010300153);安徽省中药材产业技术研发中心基金项目(ZYC20101503);安徽科技学院自然科学研究计划项目(ZRC2011294)

摘　　要：目的:比较北五味子种子和果肉中活性成分的差异。方法:采用薄层色谱法比较北五味子种子、果肉和果实中木脂素成分含量;采用苯酚-硫酸法测定种子、果肉和果实及果肉不同组分糖的含量;薄层色谱法分析三氟乙酸水解后,果肉不同组分单糖组成。结果:北五味子木脂素类主要在种子中;北五味子在果肉和种子中的总糖含量分别为169.34、5.366mg/g;果肉中各组分糖含量分别为醇溶组分117.06mg/g、醇沉组分75.67mg/g、多糖组分12.37mg/g。三氟乙酸水解后薄层分析,醇溶组分主要由葡萄糖组成,多糖组分主要由葡萄糖、半乳糖组成。结论:北五味子种子和果肉中活性成分存在明显差异。Objective： To comparatively analyze bioactive components in seeds and flesh of Schisandrae chinensis Fructus. Methods： The contents of lignan in seeds, flesh and whole fruit of Schisandrae chinensis Fructus were determined by high performance thin layer chromatography （HPTLC）. Sulfuric acid-phenol method was used to quantify sugar. After trifluoroacetic acid hydrolysis, the monosaccharide composition of the flesh was analyzed by HPTLC. Results： Lignans in Schisandrae chinensis Fructus were mainly present in the seeds, and total sugar content was 169.34 mg/g in the flesh and 5.366 mg/g in the seeds. The alcohol- soluble, alcohol-insoluble and polysaccharide components were 117.06, 75.67 mg/g and 12.37 mg/g in the fresh, respectively. The alcohol-soluble fraction was mainly composed of glucose. The polysaccharide fraction was mainly composed of glucose and galactose. Conclusion： Bioactive components reveal a significant difference between seeds and flesh of Schisandrae chinensis Fructus.

关 键 词：北五味子 种子 果肉 木脂素 多糖 

分 类 号：R284.2[医药卫生—中药学] R931.6[医药卫生—中医学]