蛹虫草废弃培养残基中虫草素的提取工艺研究  

A Study of the Technology for Extracting Cordycepin from Discarded Solid Medium of Cordyceps

作　　者：李辰[1,2] 吴盼盼[1] 卿宁[2] 梁硕 黄俊添 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006 [2]五邑大学化学与环境工程学院,广东江门529020 [3]江门市新会区现代农业发展有限公司,广东江门529100

出　　处：《五邑大学学报（自然科学版）》2012年第3期35-41,共7页Journal of Wuyi University(Natural Science Edition)

基　　金：广东省"211工程"三期重点学科建设(天然资源开发与应用平台);五邑大学博士启动项目(30613016)

摘　　要：优化并比较了微波辅助和超声波辅助提取蛹虫草培养残基中虫草素的工艺方法.采用L 9(34)正交试验设计,考察了料液比(g:mL)、乙醇含量(%)、微波功率(微波法)或提取温度(超声波法)、提取时间等4个因素对虫草素提取率的影响.结果表明:微波提取以25倍料液比、40%乙醇、240 W下微波提取1.5 min,提取2次为最佳工艺,该条件下浸膏得率为28.33%,虫草素含量为0.378 mg/g;超声波提取在45 kHz功率下,以25倍料液比、40%乙醇、60℃下超声提取30 min,提取2次为最佳工艺,该条件下浸膏得率为38.24%,虫草素含量为0.362 mg/g.比较了超声波提取2次、微波提取2次、微波—超声波联合提取以及超声—微波联合提取等方式对虫草素的提取效率,最终确定微波提取2次为最佳工艺,该工艺适用于蛹虫草培养残基中虫草素的快速高效提取.Microwave-assisted extraction （MAE） extracting cordycepin from deserted solid medium and ultrasonic-assisted extraction （UAE） for of Cordyceps were optimized and compared. The solvent quantum to solid medium （g：mL）, ethanol content （%）, microwave power （W） （in MAE） or extraction temperature （~C） （in UAE） and extraction time （rain）, etc, were reviewed using the L9（34） orthogonal design test. The extraction conditions of MAE and UAE method were both optimized. The results showed that the optimum process conditions were as follows： adding 25 folds of 40% ethanol, extracting for 1.5 min under 240 W power in MAE, two times, the extraction yield of cordycepin from the deserted solid medium was 0.378 mg/g with extractum yield at 28.33% in this process; adding 25folds of 40% ethanol, extracting for 30 min at 60 ~C in UAE, two times, the extraction yield of cordycepin from the deserted solid medium was 0.362 mg/g with extractum yield at 38.24% in this process. The MAE, UAE and eombined extraction of MAE and UAE were all developed and compared, and MAE was confirmed and chosen as the optimal method for more efficient, simpler and faster extraction of cordycepin. This developed technology is proper for the rapid and efficient extraction of cordycepin from discarded solid medium of cuhured Cordyceps.

关 键 词：蛹虫草 培养残基 虫草素 微波 超声波 

分 类 号：R914.4[医药卫生—药物化学] O657.7[医药卫生—药学]