橡实淀粉发酵生产酒精专用酵母菌株的筛选  

Screening of special yeast for ethanol production by acorn starch fermentation

作　　者：李安平[1,2] 谢碧霞[1] 沈伟[1] 蒋雅茜[1] 

机构地区：[1]中南林业科技大学,湖南长沙410004 [2]稻谷及副产物深加工国家工程实验室,湖南长沙410004

出　　处：《中南林业科技大学学报》2012年第8期98-102,共5页Journal of Central South University of Forestry & Technology

基　　金：林业公益性行业科研专项"野生木本淀粉种质资源评价与精加工技术研究"(200804033);湖南省科技计划项目"橡实淀粉生物乙醇关键技术研究"(2010NK3040)

摘　　要：从栓皮栎自然发酵基质中分离得到的36株酵母菌,经TTC平板显色图变化挑选出5株菌株,分别测试比较其发酵性能。结果表明:筛选的5株酵母菌菌落形态基本一致,但酵母菌XS03和XS04的死亡率显著低于XS01、XS02和XS05等3株酵母菌(P<0.05)的死亡率;在酒精浓度低于6%时,酵母菌XS03和XS04比酵母菌XS01、XS02和XS05等具有更高的耐酒精能力和耐高塘能力;发酵36 h后酵母XS03和XS04的失重率和出酒率显著高于XS01、XS02和XS05等3种菌种(P<0.05)。36 yeast strains has been isolated from cork oak natural fermentation matrix, of them, five strains were selected by using the TTC plate change color chart, their＇s fermentation performance were tested and compared each other. The results show that the colony morphology of the five yeast colonies were basically identical, but the yeast XS03 and XS04 mortality rate were significantly lower than the XS01, XS02 and XS05 other three yeast strains （P 〈0.05） mortality; under the condition of alcohol concentrations less than 6%, yeast XS03 and XS04 had higher alcohol tolerance and higher Gaotang resistance capacity than XS01, XS02 and XS05, etc. the weight loss rate and the yield of liquor of yeast XS03 and XS04 after being fermenting 36 hours, were significantly higher than that of XS01, XS02 and XS05 （P 〈0.05）.

关 键 词：橡实淀粉 发酵 酵母菌株 筛选 

分 类 号：S789[农业科学—林学]