乙型肝炎病毒C基因多态性及其蛋白特性分析  

Analysis of C gene polymorphism and C protein property of hepatitis B virus (HBV)

作　　者：冯娟[1] 吕艳[1] 陈昊[1] 涂建欣[1] 朱珊丽[1] 薛向阳[1] 张丽芳[1] 

机构地区：[1]温州医学院分子病毒与免疫研究所/微生物学与免疫学教研室,浙江温州325035

出　　处：《温州医学院学报》2012年第5期409-412,418,共5页Journal of Wenzhou Medical College

基　　金：国家自然科学基金资助项目(30972669)

摘　　要：目的:调查温州医学院大学生人群乙型肝炎病毒(HBV)C基因多态性及其HBc蛋白特性变化,为了解乙肝病毒核心抗原(HBcAg)变异提供理论依据。方法:采用HBV-C基因特异性引物对21份温州医学院大学生HBV携带者(血清学检测为大三阳患者)血清标本进行PCR扩增鉴定,并取19份PCR检测阳性产物直接测序;进一步用Vector NTI 8.0和DNA Star软件分析HBV-C基因多态性及HBc蛋白特性的变化。结果:21份标本中,HBV-C DNA阳性率达90.48%(19/21),与标准基因序列(Gen Bank X01587)比较,HBV-C基因同源性为93.3%～95.1%。HBV-C基因分别形成51种突变模式,其中一处由于核苷酸的改变,翻译的氨基酸也发生了相应的改变,此处氨基酸序列的第130处脯氨酸(Pro)突变为苏氨酸(Thr),其余标本均为同义突变;但突变处编码蛋白在亲水性、表面可及性及抗原性与标准株相比较无明显变化。结论:温州医学院大学生HBV携带者的HBV C基因具有多态性,但是由C基因决定的核心蛋白序列相对保守。Objective： To study the character of HBV C gene from college students in Wenzhou area, and analysis mutations of its polymorphism and protein property, providing theoretical basis of the HBV core antigen variations. Methods： The HBV DNA was extracted from the serum of HBV carriers with the HBsAg, HBcAg, HBeAg positive and anti-HBs, anti-HBe negative. The C gene of HBV was amplified with PCR by C gene specific primers from the 21 serum samples respectively, and C gene products of 19 sampls were identified by sequencing, the C gene polymorphism and HBcAg protein property were further analyzed with bio-soft of Vector NTI 8.0 and DNAstar. Results： Compared with standard C gene of HBV sequence （GenBank X01587）, gene homology was 93.3%-95.1% and 51 mutations of the C gene were found in the 19 serum samples. But there was only one amino acid mutation which was Pro to Thr at coden 130 and compared with the standard HBV C gene were not found in hydrophilicity, surface probability and antigenic index compared with the standard C protein property. Conclusion： The results show that some mutations had taken place in the nucleotide sequence of C gene of HBV obtained from the serum of HBV carriers who were college students in Wenzhou area. However, the core protein sequence determined by the C gene is relatively conservative.

关 键 词：乙型肝炎病毒 C基因 突变 多态性 

分 类 号：R37[医药卫生—病原生物学]