利用分子标记将2Ai-2染色体转移到小麦ph1b遗传背景的研究  

Transferring of the 2Ai-2 Chromosome into Wheat ph1b Genetic Background by Using Related Molecular Markers

作　　者：雷昊[1] 张悦[1] 林志珊[1] 徐琼芳[1] 叶兴国[1] 

机构地区：[1]中国农业科学院作物科学研究所/农业部作物遗传育种重点开放实验室/国家作物基因资源和基因改良重大科学工程,北京100081

出　　处：《植物遗传资源学报》2012年第5期838-842,共5页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(30571159)

摘　　要：小麦ph1b突变体可诱导部分同源染色体配对和交换,产生遗传上较为稳定、补偿性较好的重组体。将外源染色体引入ph1b的小麦遗传背景是产生目标染色体重组体的基础,但ph1b植株没有明显而稳定的表型性状,难以从表型上进行选择。本研究利用CSph1b缺失区中的分子标记Mads及外源染色体特异的分子标记P4和P68,对小麦-中间偃麦草2Ai-2(2B)异代换系N420与CSph1b的杂种F2群体及其衍生的F5株系进行ph1b-2Ai-2染色体综合体的选择,高效地获得了目标基因型。Incident of homeologous chromosome pairing and exchange often occur in hexaploid wheat with ph1b genetic background which can produce compensable and genetic stable recombinants.Therefore,transferring alien chromosomes into a wheat material with ph1b background is the basis of generating translocations between the target chromosomes.However,it is difficult to identify ph1b genotype according to its phenotype.In present study,some target plants with ph1b and the alien chromosome 2Ai-2 were selected effectively by using molecular markers Mads（on the deletion region of ph1b） for ph1b,P4 and P68（on the alien chromosome） for 2Ai-2 in the F2 population of CSph1b crossed with N420,a wheat-Thinopyrum intermedium 2B（2Ai-2）substitution line,and their derivative F5lines.

关 键 词：小麦 分子标记 PH1B 外源染色体 部分同源染色体 

分 类 号：S512.1[农业科学—作物学]