甘蔗渣多糖脱蛋白方法研究  

Study on the Deproteinization Method of Polysaccharide in Sugarcane Residue

作　　者：张斌[1] 张璐[1] 李沙沙[1] 江晓[1] 朱红霞[1] 黄志刚 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]九惠制药股份有限公司,广东惠州516007

出　　处：《辽宁中医药大学学报》2012年第10期101-102,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：广东省教育部产学研结合资助项目(2009B090300278);广州市白云区科技项目(2009-S2-41);广东省高校中药化妆品工程中心建设项目(GCZX-A1007)

摘　　要：目的:比较Sevag法、酶法对甘蔗渣多糖脱蛋白的效果。方法:以蛋白脱除率、多糖含量为考察指标,选用Sevag法和酶法对甘蔗渣多糖进行脱蛋白处理。结果:进行8次Sevag法脱蛋白后蛋白的脱除率与多糖的含量分别达到77.47%、63.6%。酶法最佳条件下蛋白脱除率达到71.84%。结论:Sevag法进行8次脱蛋白对甘蔗渣多糖脱蛋白效果最佳;酶用量2%、温度50℃、时间3h对甘蔗渣多糖脱蛋白效果最佳。Objective：To study the deproteinization by Sevag method and enzyme method of polysaccharide in Sugarcane Residue. Methods ： Taking polysaceharide removal rate and polysaccharide content as criteria, deproteinization was treated by Sevag method and enzyme method. Results ： After 8 times of deproteinization by Sevag method, polysaccharide removal rate was 77.47%, polysaccharide content was 63.6%. Polysaccharide removal rate was 71.84% by the optimum conditions of enzyme method. Conclusion： The deproteinization by Sevag method got to optimal effect after 8 times. The optimum conditions for enzyme method are as follows： enzyme concentration： 2% ; temperature： 50℃ ; time： 2h.

关 键 词：甘蔗渣 多糖 脱蛋白 正交实验 sevag法 酶法 

分 类 号：R285[医药卫生—中药学]