EGCG对食饵性肥胖幼鼠模型早期动脉病变的干预作用  

Intervention of Epigallocatechin-3 Gallate on Early Arterial Disease in Over-feeding Obese Young SD Rats

作　　者：郑绪阳[1] 李晓伟[1] 冯亚男[1] 黄先玫[1] 

机构地区：[1]杭州市第一人民医院儿科,杭州310006

出　　处：《浙江中西医结合杂志》2012年第9期678-680,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：杭州市医药卫生科技计划项目(No.2008B005)

摘　　要：目的:观察表没食子儿茶素没食子酸酯(EGCG)对肥胖幼鼠模型动脉病变和水通道蛋白7mRNA表达的干预作用。方法:通过过度喂养建立食饵性肥胖幼鼠模型,将出生第3天的雄性SD大鼠72只随机分为对照组、模型组、EGCG低剂量组和EGCG高剂量组,每组18只,HE染色观察各组腹主动脉早期动脉病变,同时运用real-time PCR测定脂肪和腹主动脉组织AQP-7mRNA表达。结果:EGCG低剂量可降低腹主动脉平滑肌中粥样斑块形成,EGCG高剂量可有效改善腹主动脉早期动脉粥样硬化形成,下调脂肪组织和腹主动脉AQP-7mRNA表达水平(P<0.01)。结论:EGCG可能通过下调肥胖大鼠脂肪组织和腹主动脉AQP-7mRNA表达水平而起到减肥和改善早期动脉粥样硬化的作用。Objective： To investigate the effect of epigallocatechin-3 gallate（EGCG） on early arterial disease and the expression of aquaporin 7（AQP-7）mRNA in obese young rats.Methods： Obese model was induced by over-feeding in young SD rats.A total of 72 3-day young rats were randomly divided into control group,model group,low-dose EGCG group,and high-dose EGCG group（18 in each）.Early abdominal aortic artery lesions were observed by HE staining.AQP-7 mRNA expression in fat and abdominal aorta tissues was determined by Real-Time PCR.Results： Lose-dose EGCG reduced the formation of atherosclerotic plaques in the abdominal aorta smooth muscles;high-dose EGCG effectively improved attenuated early atherosclerosis of the abdominal aorta and at the same time downregulated the AQP-7 mRNA expression in fat tissue and abdominal aorta as compared to the model group（P〈0.01）.Conclusions： EGCG may control the weight and attenuate early atherosclerosis of the abdominal aorta by decreasing the level of AQP-7 mRNA in adipose tissue and abdominal aorta in young obese rats.

关 键 词：肥胖幼鼠模型 表没食子儿茶素没食子酸酯 水通道蛋白7 早期动脉粥样硬化 

分 类 号：R-332[医药卫生]