用旋光法测定蜂蜜主要成分含量的研究  

The study of determining the content of compositions of in honey with optical rotation

作　　者：顾大明[1] 鲍治宇[1] 冯立彬[2] 

机构地区：[1]哈尔滨建筑大学基础科学系,黑龙江哈尔滨150090 [2]黑龙江中医药大学附属一院,黑龙江哈尔滨150040

出　　处：《哈尔滨建筑大学学报》2000年第4期124-126,共3页Journal of Harbin University of Civil Engineering and Architecture

摘　　要：测定蜜蜂中糖含量的传统方法有多种，测总含糖量的方法有硫酸－酚法、蒽酮比色法等，测定其中还原糖含量的有 3， 5－二硝基水杨酸法、斐林氏法或铁氰化钾法等。若同时测定葡萄糖、果糖和蔗糖等主要成分时则需要繁多的步骤，并且操作复杂。本文首次提出采用旋光法，根据蜂蜜主要成分 (葡萄糖、果糖和蔗糖 )的比旋光度不同，用旋光仪直接测定这些成分的含量。操作简便、结果准确。There are many methods of determining the contents of compositions in honey， but it needs many steps to determine the contents of glucose、 fructose and sucrose in honey at the same time in tradition and it is complicated to operate. Based on the rotations of glucose、 fructose and sucrose are different, the paper proposes the method with witch the rotation apparatus is used when the contents of the compositions in honey are determined.Using the rotation apparatus, it is convenient to operate and the results from the method are exact.

关 键 词：旋光度 蜂蜜 葡萄糖 果糖 蔗糖 含量 测定 

分 类 号：O657.3[理学—分析化学] S896.1[理学—化学]