影响旋光法测定2-酮基-D-葡萄糖酸含量的因素  

Factors Affecting the Determination of 2-Keto-D-gluconic Acid by Polarimetry

作　　者：郑大贵[1] 余泗莲[2] 祝显虹[1] 余彬[2] 

机构地区：[1]上饶师范学院江西省普通高校应用有机化学重点实验室,江西上饶334001 [2]百勤异VC钠有限公司,江西德兴334221

出　　处：《上饶师范学院学报》2012年第3期52-55,99,共5页Journal of Shangrao Normal University

基　　金：江西省科技条件平台建设项目(2010DTZ01900)

摘　　要：用旋光法测定发酵液中2-酮基-D-葡萄糖酸含量。详细考察了2-酮基-D-葡萄糖酸浓度、试液酸碱性和测试温度对2-酮基-D-葡萄糖酸含量测定结果的影响。结果表明,测试液中2-酮基-D-葡萄糖酸的含量在2.5~5.00g/100 mL,pH在酸性范围内,测定结果较为准确。利用有机化学原理从理论上对相关现象进行了解释。The 2 - keto - D - gluconic acid （2 - KGA） in fermentation broth was determined by polarimetry. Some fac- tom affecting the determination of 2 - KGA were investigated in detail, such as the concentration of the test solution, the temperature of the test solution and the pH of the test solution. It was found that the test solution content in 2.5-5.00 g 2- KGA per 100 mL , pH in the acidic range, more accurate measurement results could be achieved. The relevant phenomena were discussed accordinz to the relevant nfineinles of organic chemistry.

关 键 词：2-酮基-D-葡萄糖酸 旋光法 D-异抗坏血酸 

分 类 号：O656.3[理学—分析化学]