舌鳞癌Tca8113细胞株干细胞相关差异表达基因的筛选及分析  

Screen and analysis of differentially expressed genes related to stem-like cells in tongue squamous cell carcinoma Tca8113 cell line

作　　者：邹波[1,2] 季平[1,2] 孙守娟 漆小娟[1,2] 

机构地区：[1]重庆医科大学附属口腔医院颌面外科 [2]重庆市口腔疾病与生物医学研究中心,重庆401147

出　　处：《华西口腔医学杂志》2012年第5期458-462,共5页West China Journal of Stomatology

基　　金：重庆市卫生局科研基金资助项目(2008-2-232);重庆市科委自然科学基金资助项目(CSTC2005DB5241)

摘　　要：目的应用抑制消减杂交(SSH)技术筛选舌鳞癌干细胞相关差异表达基因。方法以舌鳞癌Tca8113细胞株中的醛脱氢酶(ALDH)高表达(ALDHb)r细胞与低表达(ALDHlow)细胞互为实验组和对照组,进行正反向消减杂交后建立差异表达基因文库,随机挑选差异基因克隆进行测序及生物信息学分析。结果经Blast比对分析,得到62条差异表达基因;功能聚类发现多数与细胞周期调节、细胞分化等生物行为相关;信号通路分析显示有11条基因参与的12条信号通路发生显著改变。结论应用SSH技术成功筛选出了与舌鳞癌干细胞相关的一些基因,进一步研究这些基因对探索舌鳞癌干细胞特异性表面标志物具有重要意义。Objective To screen the differentially expressed genes of stem-like ceils in tongue squamous cell car- cinoma using suppression subtractive hybridization （SSH）. Methods The cells with high and low expression of aldehyde dehydrogenase（ALDH^low and ALDH^low）were isolated respectively from human tongue squamous cell carcinoma Tca8113 cell line. SSI-I was used to identify differentially expressed genes in those two groups. Positive cell clones with high/ low-expressed ALDH were screened randomly for the genetic sequences and function analysis by the bioinformatics technology. Results Total 62 differentially expressed genes were identified, most of which got involved in regulation of cell cycle, cellular differentiation and other biology activities as gene ontology （GO） analysis indicated. Signal path- way analysis revealed 12 pathways related to 11 genes changed significantly. Conclusion Several potential novel bio- marker candidates of tongue cancer stem cells were identified and they may provide important insights into the func- tions and characteristics of human tumor stem cell.

关 键 词：舌鳞状细胞癌 肿瘤干细胞 醛脱氢酶 基因 

分 类 号：R739.86[医药卫生—肿瘤]