产油脂真菌PJX-29抗氧化活性成分研究  

The Study of Antioxidant Components from Oil Producing Fungi PJX-29

作　　者：林涛[1] 杨海燕[2] 刘天行[1] 辛志宏[1] 

机构地区：[1]南京农业大学食品科技学院,南京210095 [2]新疆农业大学食品科学与药学学院,乌鲁木齐830052

出　　处：《中国粮油学报》2012年第9期53-59,共7页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金(31071586);中央高校基本科研业务费专项(KYZ201118)

摘　　要：对一株红树林来源的产油脂真菌PJX-29发酵产物中的抗氧化成分进行分离和鉴定。利用溶剂萃取、硅胶柱色谱及制备HPLC等分离手段从发酵产物中分离得到2个甘油酯类化合物和其他5个化合物,通过理化性质分析及波谱学方法鉴定其化学结构分别为:十五碳单甘油酯,9″,12″-十七碳二烯酸,十六烷基甘油酯,6-戊基-α-吡喃酮4,,6-二羟基-9,10-二甲基苯乙酮,双(8-8'-7羟基-4-甲氧基-5-甲基香豆素),对羟基苯甲酸6,-氨基苯甲醛,并以DPPH法评价了化合物的抗氧化活性,结果表明化合物1,化合物2和化合物6具有较强的抗氧化活性,其IC50分别为:(11.38±0.61)(、9.33±0.80)和(11.12±1.01)μg/mL。The antioxidant components duction fungus PJX -29 derived from Ocean were isolated and identified from the fermentation product of the Oil pro- Mangroves. Two glyceride compounds and other five compounds were iso- lated with solvent extraction, silica gel column chromatography and preparative HPLC and their structures were identi- fied as monoglycerides ,9＂, 12＂ - Heptadecadienoic acid, 1 - （hydroxymethyl） - 2 - [ （ 1 - oxohexadecyl） oxy ] ethyl ester,6 - Pentyl - ct - pyrone ,4,6 - dihydroxy - 9,10 - dimethylacetophenone, bis [ 8 - 8＇ - （7 - hydroxy - 4 - me- thoxy -5 -methylcoumarin） ] , Hydroxybenzoic acid ,6 -Aminobenzaldehyde by physicochemical properties and spec- tral analyses, respectively. The antioxidant activity of the compounds were evaluated by DPPH method, the result showed that compounds 1,2 and 6 have potential antioxidant activity, and their ICs0 are （ 11.38 ± 0.61 ）, （9.33 ± 0.80） and （ 11.12 ± 1.01 ） μg/mL,respectively.

关 键 词：产油脂真菌 发酵产物 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]