不同因素对夏枯草总黄酮稳定性及DPPH自由基清除活性的影响  

Effect of different factors on the stability and DPPH free radical scavenging activity of Prunella vulgaris L flavonoids

作　　者：闫家凯[1] 张国文[1] 周佳[1] 胡明明[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《南昌大学学报（理科版）》2012年第4期341-346,共6页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(31060210);江西省自然科学基金资助项目(20114BAB204019);江西省教育厅科技资助项目(GJJ11287)

摘　　要：利用紫外分光光度法研究了温度、光照、pH值对夏枯草黄酮稳定性的影响,及以夏枯草黄酮清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基的活性变化为指标,研究了温度、光照、pH值、还原剂、氧化剂、食品成分、防腐剂及金属离子对夏枯草黄酮DPPH自由基清除活性的影响。结果表明:高温、光照对夏枯草黄酮有一定的降解作用且使其对DPPH自由基的清除活性降低;还原剂Na2SO3、食品成分葡萄糖、防腐剂苯甲酸钠、金属离子(Cu2+、Mg2+)对夏枯草黄酮DPPH自由基清除活性的影响较大,而氧化剂H2O2的影响相对较小;夏枯草黄酮在pH值为6和7时较稳定,活性保存率较高。The effect of temperature,light,pH on the stability of Prunella vulgaris L flavonoids was studied with Ultraviolet spectrophotometry.The change of scavenging activity on DPPH free radical of Prunella vulgaris L flavonoids was selected as the index to investigate the effects of temperature,light,pH,oxidizing agent,reducing agent,food materials,preservative,metal ions on DPPH free radical scavenging activity of Prunella vulgaris L flavonoids.The results showed that high temperature with light have a little degradation on Prunella vulgaris L flavonoids and reduce its activity,while reducing agent Na2SO3,food materials glucose,preservative sodium benzoate and Cu2＋ or Mg2＋ have significant effect on DPPH free radical scavenging activity of Prunella vulgaris L flavonoids.The effect of oxidizing agent H2O2 is relatively smaller.The antioxidant activity of Prunella vulgaris L flavonoids is stable under the pH for six and seven.

关 键 词：夏枯草黄酮 1 1-二苯基-2-三硝基苯肼(DPPH)自由基 稳定性 清除活性 

分 类 号：TS207.3[轻工技术与工程—食品科学]