诱变选育以菊芋为原料高产甘露醇的乳酸菌  

Mutation breeding of lactic acid bacteria for high yield of mannitol from saccharified Jerusalem artichoke

作　　者：岳敏[1,2] 曹海龙[1] 李曙光[1] 杨凤丽[1,2] 张建平[1,2] 杜昱光[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023 [2]中国科学院研究生院,北京100049

出　　处：《中国酿造》2012年第9期53-56,共4页China Brewing

基　　金：国家高技术研究发展计划‘863计划’项目(2012AA021205);国家科技支撑计划项目(2012BAD32B09);中国科学院知识创新工程重要方向项目(No.KSCX2-EW-G-5)

摘　　要：以短乳杆菌为出发菌株,采用大气压冷等离子体介质阻挡放电法和离子束对乳酸菌进行诱变。以菊芋作为筛选培养基,摇瓶筛选得到高产甘露醇的菌株。之后对筛选出的高产甘露醇菌株进行遗传稳定性实验,证实该菌株遗传稳定。在5L发酵罐中进行发酵,该菌株可有效地将果糖转化为甘露醇,产率达到62%。这株诱变菌可作为高产甘露醇的优良菌株。Mannitol is the main product when fructose is used as the substrate for heterofermentative lactic acid bacteria under anaerobic conditions. In this study, the mutation of a Lactobacillus brevis strain was induced by atmospheric pressure dielectric barrier discharge plasma and ion beam to im- prove its mannitol productivity. A positive mutant was selected for its superior mannitol productivity when saccharified Jerusalem artichoke juice was used in selective medium. And it was also confirmed that the mutant strain had high genetic stability. When fermented in 5L fermentor, the mutant could effectively convert fi＇uctose into mannitol and the yield of marmitol reached 62%. It was indicated that the mutant strain could be a candidate for industrial mannitol production.

关 键 词：大气压介质阻挡放电 离子束 菊芋 甘露醇 乳酸菌 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]