高温厌氧菌CBZ-S-MEE2利用不同碳源发酵产氢的研究  

Hydrogen production from various carbon sources fermented by a thermophilic anaerobic bacterium CBZ-S-MEE2

作　　者：于雷[1] 高猛[1,2] 张作明[2] 任浩[3] 张凤[3] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]吉林大学生命科学学院分子酶学工程教育部重点实验室,吉林长春130112 [3]吉林大学化学学院超分子结构与材料国家重点实验室,吉林长春130112

出　　处：《中国酿造》2012年第9期66-70,共5页China Brewing

基　　金：吉林省科技发展计划项目(20100563)

摘　　要：以从长白山温泉中分离的一株高温厌氧产氢菌CBZ-S-MEE2为菌种,对该菌利用不同碳源发酵产氢进行研究。利用葡萄糖进行发酵产氢时,最大产气速率为180mL(/L.h),产氢效率为1.8mol H2/mol葡萄糖,表明该菌可以快速利用葡萄糖发酵产氢,对葡萄糖的转化效率高。对未经预处理纤维素和以纤维素为主要成分的碳源进行发酵时,该菌在硫元素缺失或pH偏碱性的状态下不能充分的生长,产氢速率低。在改进试验中,通过维持较好的硫元素平衡,以纤维素为唯一碳源发酵时,最大产气速率为77mL(/L.h),产氢效率为7.5mol H2/gVS。表明高温厌氧菌CBS-Z-MEE2可以利用未经预处理的纤维素原料为唯一碳源进行发酵产氢,这为以纤维素为主要成分的生物质发酵产氢奠定了技术基础。A thermophilic anaerobic hydrogen-producing bacterium CBZ-S-MEE2 was isolated from the hot springs of Changbai Mountain. The hydrogen production from various carbon sources fermented by CBZ-S-MEE2 was studied. On glucose, the maximum hydrogen production rate was 180ml/（L.h）, and the efficiency of hydrogen production was 1.8mol H2/mol glucose. The results indicated that CBZ-S-MEE2 converted glucose to Hz fast and effectively. When untreated cellulose or the substrate mainly consisting of cellulose was used as carbon source for hydrogen fermentation, CBZ-S-MEE2 could grow well with the lack of sulfur or basic pH, and the hydrogen production rate was low. In further experiment, abundant sulfur was added in the medium, then the maximum hydrogen production rate and hydrogen production efficiency reached 77mL/（L-h） and 7.5mol H2/gVS respectively using untreated cellulose as sole carbon source. It was demonstrated that CBS-Z-MEE2 could use untreated cellulose as sole carbon source for hydrogen production which was critical for hydrogen production from cellulose by microbial fermentation.

关 键 词：产氢 高温厌氧菌 纤维素 碳源 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]