胰腺癌患者p16基因序列分析  

Analysis of p16 Gene in Pancreatic Carcinoma

作　　者：周家华[1] 杨德同[1] 张丽珊[2] 苏占海[2] 

机构地区：[1]南京铁道医学院附属医院胆胰外科,江苏南京210009 [2]南京铁道医学院生物学教研室,江苏南京210009

出　　处：《南京铁道医学院学报》2000年第2期94-97,共4页Journal of Nanjing Railway Medical College

摘　　要：目的 :研究p16基因的突变在人胰腺癌发生中的作用。方法 :应用PCR、PCR SSCP、DNA测序 ,研究p16基因的突变情况。结果 :在 3 5例人胰腺癌标本中 ,发现有 12例在p16基因第 2外显子部分存在至少 5 2 2bp的纯合缺失。另有7例存在 2个点突变 ,突变位点均相同 ,一为第 12 6密码子碱基GTC突变为AAT ,氨基酸由缬氨酸 (Val)变成了天冬酰胺(Asn) ,全为错义突变 ;另一为第 12 7密码子GCA→GCG的同义突变。结论Objective The experiment was designed to study the role of genetic mutation of p16 in pathogenesis of pancreatic carcinoma.Methods The mutation of p16 was analyzed by using polymerase chain reaction(PCR),single strand conformation polymorphism(SSCP),DNA sequencing.Result Out of 35 cases,12 cases had at least 522?bp homozygous deletion in exon 2 and 7 had two point mutations.One of them is a missense mutation at codon 126 GTC→AAT(Val126→Asn),the other is a same sense mutation at codon 127 GCA→GCG (Ala127Ala).Conclusion The mutation of p16 has a higher incidence in pancreatic carcinoma.

关 键 词：胰腺癌 P16基因 突变 病理 

分 类 号：R735.9[医药卫生—肿瘤]