上皮源性恶性肿瘤患者p16抑癌基因甲基化研究  

METHYLATION OF THE P16/MTS1 GENE IN PATIENTS WITH EPITHELIOGENIC MALIGNANT TUMOR

作　　者：肖白[1] 朱章菱[1] 刘敬忠[1] 王跃[1] 迟闺珠[1] 于莎莎[1] 刘复兴 李正廷[1] 张愚[1] 高居忠[1] 

机构地区：[1]首都医科大学附属北京红十字朝阳医院,北京100020

出　　处：《肿瘤研究与临床》2000年第3期147-149,共3页Cancer Research and Clinic

摘　　要：目的 :了解中国人群上皮源性恶性肿瘤患者 p16基因甲基化异常状况。方法 :收集 131例肺、食管、卵巢、子宫内膜、膀胱、喉、鼻咽癌患者肿瘤组织 ,甲基化敏感限制性内切酶 Sma I消化 DNA,PCR扩增 p16基因外显子 1,分析 5′Cp G岛异常甲基化。结果 :131例患者中 p16基因甲基化异常检出率为 19.1%。甲基化频率较高的肿瘤类型是鼻咽癌 (2 7.9% )、膀胱癌 (2 5 .0 % )和肺癌 (2 5 .0 % )。结论 :在中国人群中上皮源性恶性肿瘤患者中 ,p16基因异常甲基化是较为频繁发生的基因变化 ,可能与病理分级和预后有关。Objective:To approach methylation status of the p16 gene in Chinese patients with neoplasms.Methods:131 DNAs from patients with primary tumor were digested by methylation sensitive restriction enzyme Sma Ⅰ,and Exon 1 of the p16 gene was amplified in the SmaⅠ digested DNAs.Methylation in the 5′CpG island of the p16 gene was analysed.Results:.Aberrant methylation of the p16 gene was found in 25(19.1%) of 131 primary tumors. Primary tumors of the nasopharyngeal carcinoma(27.9%),bladder cancer(25.0%) and lung cancer(25.0%) displayed higher rates of methylation of this CpG island.Conclusion:Methylation of the p16 gene is a common event in Chinese patients with malignant tumors,and may be associated with histological grade and prognosis.

关 键 词：p16/MTS1抑癌基因 甲基化 上皮源性恶性肿瘤 

分 类 号：R730.2[医药卫生—肿瘤]