痛风宁部分提取物对黄嘌呤氧化酶的抑制作用  

Inhibitory Effects of Some Extracts in Tongfengning on Xanthine Oxidase

作　　者：王晓梅[1] 赵岩[2] 赵卫星[1] 

机构地区：[1]宝鸡文理学院化学化工系,陕西宝鸡721013 [2]宝鸡市中医医院,陕西宝鸡721001

出　　处：《安徽中医学院学报》2012年第5期56-58,共3页Journal of Anhui Traditional Chinese Medical College

摘　　要：目的观察痛风宁部分提取物对黄嘌呤氧化酶(xanthine oxidase,XOD)的影响及抗氧化作用。方法通过XOD催化黄嘌呤产生尿酸,采用铁氰化钾还原法测定还原力,分别测定各提取物对反应体系吸光度的影响。结果秦皮乙酸乙酯提取物和秦皮乙醇提取物及威灵仙乙酸乙酯提取物对XOD的抑制作用最强,威灵仙乙酸乙酯提取物的还原能力最强。结论痛风宁部分提取物具有较强的抑制XOD活性作用并具有较强的体外抗氧化活性。Objective To observe the inhibitory effects of some extracts in Tongfengning, a traditional Chinese medicine prescription for treating gout, on xanthine oxidase （XOD） and their antioxidant activity. Methods Uric acid was produced from xanthine in the presence of XOD. The reducing power was evalua ted by the potassium ferricyanide reduction method, and the impacts of the extracts on the absorbance of the reaction system were determined. Results The ethyl acetate extract and ethanol extract of ash bark and the ethyl acetate extract of Chinese Clematis root showed the strongest inhibitory effects on XOD. The ethyl acetate extract of Chinese Clematis root showed the strongest reducing power. Conclusion Some ex tracts in Tongfengning have strong inhibitory effects on XOD and strong antioxidant activity in vitro.

关 键 词：痛风 痛风宁 提取物 黄嘌呤氧化酶 还原力 秦艽 秦皮 威灵仙 

分 类 号：R285.5[医药卫生—中药学]