高效液相色谱法测定麦精中的低聚糖  

Determination of oligosaccharide in malt extract by HPLC method

作　　者：吕宝龙[1] 陈如琪[1] 卢艳花[1] 刘少伟[1] 

机构地区：[1]华东理工大学生物工程学院,上海200237

出　　处：《食品科技》2012年第10期257-259,263,共4页Food Science and Technology

基　　金：中央高校基本科研业务费专项资金;上海科委对本研究的资助课题(10dz1200904)

摘　　要：采用高效液相色谱法对麦精中的纤维三糖和纤维四糖进行了定量测定研究,最终确定的最佳分析方法为CarbomixH-NP10:5%分析柱;示差折光检测器55℃柱温;流动相:2.5mmol/LH2SO4水溶液;流速:0.5mL/min;进样量:20μL。该方法精密度RSD<2%,回收率>97%。在此色谱条件下15min内完成了三糖和四糖的分离,结果比较理想。研究表明此新建方法准确而快速,并能适用于绝大部分食品中低聚糖的测定。HPLC was established to determine cellotriose and cellotetrose in malt extract. The final chromatographic conditions are as follows： Carbomix H-NP10： 5% chromatographic column with refractive index detector, elution influent is 2.5 mmol/L sulfuric acid solution, flow rate 0.5 mL/min and injection volume 20 IJL. The repeatability relative standard deviations of this method is below 2% and the recovery is over 97%. A satisfied result is obtained under this chromatographic conditions, and the whole separation can be finished within 15 min. The results showed that this new HPLC analysis method is not only correct but also fast enough to be used to determine most kinds of foods containing these oliqosaccharide.

关 键 词：麦精 高效液相色谱法 纤维三糖 纤维四糖 

分 类 号：TS207.3[轻工技术与工程—食品科学]